A perivascular origin for mesenchymal stem cells in multiple human organs

被引：3003

作者：

Crisan, Mihaela ^{[1
,2
]}

Yap, Solomon ^{[1
,2
]}

Casteilla, Louis ^{[1
,2
,4
]}

Chen, Chien-Wen ^{[1
,2
]}

Corselli, Mirko ^{[1
,2
]}

Park, Tea Soon ^{[1
,2
]}

Andriolo, Gabriella ^{[3
]}

Sun, Bin ^{[1
,2
]}

Zheng, Bo ^{[1
,2
]}

Zhang, Li ^{[5
]}

Norotte, Cyrille ^{[1
,2
]}

Teng, Pang-Ning ^{[1
,2
]}

Traas, Jeremy ^{[6
]}

Schugar, Rebecca ^{[1
,2
]}

Deasy, Bridget M. ^{[1
,2
]}

Badylak, Stephen ^{[5
]}

Buehring, Hans-Joerg ^{[7
]}

Giacobino, Jean-Paul ^{[1
,2
]}

Lazzari, Lorenza ^{[3
]}

Huard, Johnny ^{[1
,2
]}

Peault, Bruno ^{[1
,2
,5
]}

机构：

[1] Childrens Hosp Pittsburgh, Dept Pediat, Stem Cell Res Ctr, Pittsburgh, PA 15213 USA

[2] Childrens Hosp Pittsburgh, Dept Orthopaed Surg, Stem Cell Res Ctr, Pittsburgh, PA 15213 USA

[3] Fdn Osped Maggiore Policlin, Dept Regenerat Med, I-20122 Milan, Italy

[4] CNRS, UPS, UMR 5018, F-31059 Toulouse, France

[5] McGowan Inst Regenerat Med, Pittsburgh, PA 15213 USA

[6] Childrens Hosp Philadelphia, Div Surg, Philadelphia, PA 19104 USA

[7] Univ Clin Tubingen, Dept Internal Med, D-72076 Tubingen, Germany

来源：

CELL STEM CELL | 2008年 / 3卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.stem.2008.07.003

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-R beta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.

引用

页码：301 / 313

页数：13

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