Reduced synchrony of Ca2+ release with loss of T-tubules -: a comparison to Ca2+ release in human failing cardiomyocytes

Objectives: During cardiac excitation-contraction coupling, Ca2+ release from the sarcoplasmic reticulum (SR) occurs at the junctional complex with the T-tubules, containing the L-type Ca2+ channels. A partial loss of T-tubules has been described in myocytes from failing canine and human hearts. We examined how graded reduction of T-tubule density would affect the synchrony of Ca2+ release. Methods: Adult pig ventricular myocytes were isolated and cultured for 24 and 72 h. T-tubules, visualized with di-8-ANEPPS, and [Ca2+](i) transients (Fluo-3) were recorded during confocal line scan imaging. Results: Cultured cardiomyocytes exhibited a progressive reduction in T-tubule density. [Ca2+](i) transients showed small areas of delayed Ca2+ release which gradually increased in number and size with loss of T-tubules. Local [Ca2+](i) transients in the delayed regions were reduced. Due to these changes, loss of T-tubules resulted in an overall slowing of the rise of [Ca2+] along the entire line scan and transient magnitude tended to be reduced, but there was no change in SR Ca2+ content. Human myocytes isolated from failing hearts had a T-tubule density comparable to that of freshly isolated pig milocytes. The size, but not the number, of delayed release areas tended to be larger. The overall rate of rise of [Ca2+](i) was significantly faster than in pig myocytes with low T-tubule density. Conclusions: Loss of T-tubules reduces the synchrony of SR Ca2+ release. This could contribute to reduced efficiency of excitation-contraction coupling in heart failure, though dyssynchrony in human failing cells appears to be modest. (C) 2004 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.