Analytical and semipreparative enantiomeric separation of azole antifungal agents by high-performance liquid chromatography on polysaccharide-based chiral stationary phases - Application to in vitro biological studies

被引：18

作者：

Cirilli, R

Costi, R

Di Santo, R

Ferretti, R

La Torre, F

Angiolella, L

Micocci, A

机构：

[1] Ist Super Sanita, Lab Chim Farm, I-00161 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Studi Farmaceut, Fdn Cenci Bolognetti, Inst Pasteur, I-00185 Rome, Italy

[3] Univ Roma La Sapienza, Ist Microbiol, I-00185 Rome, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 942卷 / 1-2期

关键词：

enantiomeric separation; chiral stationary phases; LC; azoles;

D O I：

10.1016/S0021-9673(01)01401-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid chromatography (HPLC) was used for the enantiomeric separation of chiral imidazole derivatives endowed with antimycotic activity. Enantioselective columns, containing carbamates of cellulose and amylose, were used. The influence of the nature and content of an alcoholic modifier in the mobile phase was studied. The isolated enantiomers, separated on semipreparative columns, were submitted to in vitro biological investigations, (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：107 / 114

页数：8

共 12 条

[1] ANTIFUNGAL AGENTS .9. 3-ARYL-4-[ALPHA-(1H-IMIDAZOL-1-YL)ARYLMETHYL]PYRROLES - A NEW CLASS OF POTENT ANTICANDIDA AGENTS [J].