Creating conditional mutations in mammals

被引:47
作者
Spencer, DM
机构
[1] Dept. of Microbiolgy and Immunology, Baylor College of Medicine, Houston
关键词
D O I
10.1016/0168-9525(96)10013-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
For many years geneticists have relied on conditional activation to study the roles of cytolytic or cytostatic Proteins in lower eukaryotes or to elucidate the epistatic relationships of proteins in a signaling pathway. Regardless of their merits, conditional mutants have only been available to mammalian geneticists within the past few years, Moreover, the approaches that exist are still limited to a subset of signaling molecules and are not reliable for in vivo studies. Now, added to the biologist's toolbox is a versatile new approach based on chemically induced dimerization that should allow for the reversible regulation of proteins at the cell surface, intracellularly, in the nucleus and in vivo.
引用
收藏
页码:181 / 187
页数:7
相关论文
共 31 条
[1]   RATIONAL DESIGN OF ORTHOGONAL RECEPTOR-LIGAND COMBINATIONS [J].
BELSHAW, PJ ;
SCHOEPFER, JG ;
LIU, KQ ;
MORRISON, KL ;
SCHREIBER, SL .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (19) :2129-2132
[2]  
BELSHAW PJ, IN PRESS P NATL ACAD
[3]   HEAT-INDUCIBLE DEGRON - A METHOD FOR CONSTRUCTING TEMPERATURE-SENSITIVE MUTANTS [J].
DOHMEN, RJ ;
WU, PP ;
VARSHAVSKY, A .
SCIENCE, 1994, 263 (5151) :1273-1276
[4]   A NOVEL GENETIC SYSTEM TO DETECT PROTEIN PROTEIN INTERACTIONS [J].
FIELDS, S ;
SONG, OK .
NATURE, 1989, 340 (6230) :245-246
[5]   TIGHT CONTROL OF GENE-EXPRESSION IN MAMMALIAN-CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS [J].
GOSSEN, M ;
BUJARD, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5547-5551
[6]   TRANSCRIPTIONAL ACTIVATION BY TETRACYCLINES IN MAMMALIAN-CELLS [J].
GOSSEN, M ;
FREUNDLIEB, S ;
BENDER, G ;
MULLER, G ;
HILLEN, W ;
BUJARD, H .
SCIENCE, 1995, 268 (5218) :1766-1769
[7]  
Gossen Manfred, 1994, Current Opinion in Biotechnology, V5, P516, DOI 10.1016/0958-1669(94)90067-1
[8]   ANTISENSE HAS GROWING PAINS [J].
GURA, T .
SCIENCE, 1995, 270 (5236) :575-577
[9]   SIGNAL-TRANSDUCTION IN T-LYMPHOCYTES USING A CONDITIONAL ALLELE OF SOS [J].
HOLSINGER, LJ ;
SPENCER, DM ;
AUSTIN, DJ ;
SCHREIBER, SL ;
CRABTREE, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (21) :9810-9814
[10]   BIOCHEMICAL GENETICS OF NEUROSPORA [J].
HOROWITZ, NH .
ADVANCES IN GENETICS, 1950, 3 :33-71