Detection and characterization of cerein 7, a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity

被引:41
作者
Oscáriz, JC [1 ]
Lasa, I [1 ]
Pisabarro, AG [1 ]
机构
[1] Univ Publ Navarra, Dept Prod Agr, Pamplona 31006, Spain
关键词
bacteriocin; cerein; peptidic antibiotic; Bacillus cereus;
D O I
10.1111/j.1574-6968.1999.tb08696.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A bacteriocin-producing strain of Bacillus cereus was identified and isolated from a soil sample. The bacteriocin could be purified by a two-step procedure: ammonium sulfate precipitation of culture supernatants followed by a butanol extraction step, the antibiotic was recovered from the organic phase. The peptidic nature of the bacteriocin was proven by its sensitivity to proteolytic enzymes; its molecular mass, determined by mass spectrometry, was 3940 Da; and its amino-terminal sequence (GWGDVL) is unique in the databases. The compound was active against most Gram-positive but not Gram-negative bacteria. This is to our knowledge the first bacteriocin with these characteristics reported to be produced by B. cereus. (C) 1999 Federation of European Microbiological Societies.
引用
收藏
页码:337 / 341
页数:5
相关论文
共 14 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   ISOLATION AND CHARACTERIZATION OF PEDIOCIN L50, A NEW BACTERIOCIN FROM PEDIOCOCCUS-ACIDILACTICI WITH A BROAD INHIBITORY SPECTRUM [J].
CINTAS, LM ;
RODRIGUEZ, JM ;
FERNANDEZ, MF ;
SLETTEN, K ;
NES, IF ;
HERNANDEZ, PE ;
HOLO, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2643-2648
[3]  
DAESCHEL M A, 1990, Food Microbiology (London), V7, P91, DOI 10.1016/0740-0020(90)90014-9
[4]   Industry perspectives on the use of natural antimicrobials and inhibitors for food applications [J].
Gould, GW .
JOURNAL OF FOOD PROTECTION, 1996, :82-86
[5]   BACTERIOCINS OF GRAM-POSITIVE BACTERIA [J].
JACK, RW ;
TAGG, JR ;
RAY, B .
MICROBIOLOGICAL REVIEWS, 1995, 59 (02) :171-200
[6]   SDS PAGE STRONGLY OVERESTIMATES THE MOLECULAR MASSES OF THE NEUROFILAMENT PROTEINS [J].
KAUFMANN, E ;
GEISLER, N ;
WEBER, K .
FEBS LETTERS, 1984, 170 (01) :81-84
[7]  
KLAENHAMMER TR, 1993, FEMS MICROBIOL REV, V12, P39, DOI 10.1111/j.1574-6976.1993.tb00012.x
[8]   GENES INVOLVED IN SELF-PROTECTION AGAINST THE LANTIBIOTIC SUBTILIN PRODUCED BY BACILLUS-SUBTILIS ATCC-6633 [J].
KLEIN, C ;
ENTIAN, KD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (08) :2793-2801
[9]   TRANSDUCTION OF LINKED GENETIC CHARACTERS OF THE HOST BY BACTERIOPHAGE-PL [J].
LENNOX, ES .
VIROLOGY, 1955, 1 (02) :190-206
[10]  
Mayr H. A., 1972, METHODS MICROBIOLO A, P315, DOI DOI 10.1016/S0580-9517(08)70618-4