An improved method for MN genotyping by the polymerase chain reaction

被引：12

作者：

Nakayashiki, N ^{[1
]}

Sasaki, Y ^{[1
]}

机构：

[1] IWATE PREFECTURAL POLICE HEADQUARTERS,SCI CRIME DETECT LAB,MORIOKA,IWATE 020,JAPAN

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1996年 / 109卷 / 04期

关键词：

MN blood group system; genotyping; forensic evidence;

D O I：

10.1007/BF01225522

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

a novel method of human MN blood group genotyping is reported using the polymerase chain reaction. Genotyping is based on two base substitutions characteristic of M and N alleles in the 2nd exon of the glycophorin A gene. Using a newly designed primer trio, PCR products for M (255 bp) and N (270 bp) alleles are rapidly and simultaneously detected by a single PCR procedure and subsequent polyacrylamide gel electrophoresis. This method enables MN genotyping from not only minute but also degraded DNA samples.

引用

页码：216 / 217

页数：2

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