Production of a monoclonal antibody and development of an immunoassay for detection of Cr(III) in water samples

被引：25

作者：

Zhou, Y. ^{[1
]}

Li, Y. S. ^{[1
]}

Meng, X. Y. ^{[1
]}

Zhang, Y. Y. ^{[1
]}

Yang, L. ^{[1
]}

Li, Z. H. ^{[1
]}

Zhang, J. H. ^{[1
]}

Wang, X. R. ^{[1
]}

Liu, J. Q. ^{[2
]}

Lu, S. Y. ^{[1
]}

Ren, H. L. ^{[1
]}

Hu, P. ^{[1
]}

Liu, Z. S. ^{[1
]}

机构：

[1] Jilin Univ, Key Lab Zoonosis, Minist Educ, Inst Zoonosis,Coll Vet Med, Changchun 130062, Peoples R China

[2] Prod Qual Test Inst Jilin Prov, Changchun 130022, Peoples R China

来源：

CHEMOSPHERE | 2013年 / 93卷 / 10期

关键词：

Mab; Assay; Cr(III); Complete antigen; SELECTIVE DETECTION; GOLD NANOPARTICLES; HEAVY-METALS; CHROMIUM; ASSAY; CR(VI); PROBE; SPECIATION; CD(II); PB(II);

D O I：

10.1016/j.chemosphere.2013.08.088

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

In this study we report the production of a monoclonal antibody (Mab) specific for Cr(III)-chelate and the development of a competitive immunoassay for detection of Cr(III) in water samples. In the assay, the complete antigen (Cr(III)-ITCBE-BSA) was used as coating antigen, and Cr(III)-ITCBE as competitor competes with coating antigen to bind with Mab. Using this approach, the spiked water samples with Cr(III) were detected. The linear range of the detection was 0.7-12.4 ng mL(-1). The limit of the detection (LOD) was 0.51 ng mL(-1). The spiked results were also confirmed by ICP-MS, which showed a good correlation (R-2 = 0.997) between the two methods. The results indicated that the developed assay was reliable and suitable for the detection of Cr(III) in water samples. (C) 2013 Elsevier Ltd. All rights reserved.

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收藏

页码：2467 / 2472

页数：6

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