Construction of a novel virus that targets HIV-1-infected cells and controls HIV-1 infection

被引：184

作者：

Schnell, MJ

Johnson, JE

Buonocore, L

Rose, JK

机构：

[1] YALE UNIV,SCH MED,DEPT PATHOL,NEW HAVEN,CT 06510

[2] YALE UNIV,SCH MED,DEPT CELL BIOL,NEW HAVEN,CT 06510

[3] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510

来源：

CELL | 1997年 / 90卷 / 05期

关键词：

D O I：

10.1016/S0092-8674(00)80350-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a recombinant vesicular stomatitis virus lacking its glycoprotein gene and expressing instead the HIV-1 receptor CD4 and a coreceptor, CXCR4. This virus was unable to infect normal cells but did infect, propagate on, and kill cells that were first infected with HIV-1 and therefore had the HIV membrane fusion protein on their surface. Killing of HIV-l-infected cells controlled HIV infection in a T cell line and reduced titers of infectious HIV-1 in the culture by as much as 10(4)-fold. Such a targeted virus could have therapeutic value in reducing HIV viral load. Our results also demonstrate a general strategy of targeting one virus to the envelope protein of another virus to control infection.

引用

收藏

页码：849 / 857

页数：9

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