Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis

被引:21
作者
Jones, TL
Karavanova, I
Chong, L
Zhou, RP
Daar, IO
机构
[1] NCI,FREDERICK CANC RES & DEV CTR,LAB LEUKOCYTE BIOL,FREDERICK,MD 21702
[2] NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702
[3] RUTGERS STATE UNIV,CANC RES LAB,PISCATAWAY,NJ 08855
关键词
Eph ligand; xenopus development; nervous system;
D O I
10.1038/sj.onc.1201082
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and characterized the first Xenopus transmembrane Eph ligand, XLerk (Xenopus Ligand for Eph Receptor Tyrosine Kinases). While this ligand has 72% identity with the closest mammalian family member, Lerk-2, it is the cytoplasmic domain of this molecule that is the most conserved domain with 95% identity, XLerk exists as a maternally expressed mRNA, however, expression of transcripts and protein increase during gastrulation and again in the late swimming tadpole stage. In the adult, XLerk is expressed at low levels in mast adult tissues with increased levels observed in the kidney, oocytes, ovary and testis. While low levels of XLerk expression are observed in the adult brain, ill situ hybridization analysis demonstrates prominent expression in the developing olfactory system, retina, hindbrain, cranial ganglia, and somites, Furthermore, we have shown that XLerk transcripts are significantly elevated during mesoderm induction caused by activin and FGF, but not during noggin-induced neuralization. These results suggest a role for XLerk in the developing mesenchymal and nervous tissue.
引用
收藏
页码:2159 / 2166
页数:8
相关论文
共 57 条
[1]   B61 IS A LIGAND FOR THE ECK RECEPTOR PROTEIN-TYROSINE KINASE [J].
BARTLEY, TD ;
HUNT, RW ;
WELCHER, AA ;
BOYLE, WJ ;
PARKER, VP ;
LINDBERG, RA ;
LU, HS ;
COLOMBERO, AM ;
ELLIOTT, RL ;
GUTHRIE, BA ;
HOLST, PL ;
SKRINE, JD ;
TOSO, RJ ;
ZHANG, M ;
FERNANDEZ, E ;
TRAIL, G ;
VARNUM, B ;
YARDEN, Y ;
HUNTER, T ;
FOX, GM .
NATURE, 1994, 368 (6471) :558-560
[2]   MOLECULAR CHARACTERIZATION OF A FAMILY OF LIGANDS FOR EPH-RELATED TYROSINE KINASE RECEPTORS [J].
BECKMANN, MP ;
CERRETTI, DP ;
BAUM, P ;
VANDENBOS, T ;
JAMES, L ;
FARRAH, T ;
KOZLOSKY, C ;
HOLLINGSWORTH, T ;
SHILLING, H ;
MARASKOVSKY, E ;
FLETCHER, FA ;
LHOTAK, V ;
PAWSON, T ;
LYMAN, SD .
EMBO JOURNAL, 1994, 13 (16) :3757-3762
[3]   MOLECULAR-CLONING OF A LIGAND FOR THE EPH-RELATED RECEPTOR PROTEIN-TYROSINE KINASE HTK [J].
BENNETT, BD ;
ZEIGLER, FC ;
GU, QM ;
FENDLY, B ;
GODDARD, AD ;
GILLETT, N ;
MATTHEWS, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (06) :1866-1870
[4]  
BERGEMANN AD, 1995, MOL CELL BIOL, V15, P4921
[5]   Cell-cell adhesion mediated by binding of membrane-anchored ligand LERK-2 to the EPH-related receptor human embryonal kinase 2 promotes tyrosine kinase activity [J].
Bohme, B ;
Vandenbos, T ;
Cerretti, DP ;
Park, LS ;
Holtrich, U ;
RubsamenWaigmann, H ;
Strebhardt, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (40) :24747-24752
[6]   EFFICIENT CLONING OF CDNAS OF RETINOIC ACID-RESPONSIVE GENES IN P19 EMBRYONAL CARCINOMA-CELLS AND CHARACTERIZATION OF A NOVEL MOUSE GENE, STRA1 (MOUSE LERK-2/EPLG2) [J].
BOUILLET, P ;
OULADABDELGHANI, M ;
VICAIRE, S ;
GARNIER, JM ;
SCHUHBAUR, B ;
DOLLE, P ;
CHAMBON, P .
DEVELOPMENTAL BIOLOGY, 1995, 170 (02) :420-433
[7]   MEMBRANE-BOUND LERK2 LIGAND CAN SIGNAL THROUGH 3 DIFFERENT EPH-RELATED RECEPTOR TYROSINE KINASES [J].
BRAMBILLA, R ;
SCHNAPP, A ;
CASAGRANDA, F ;
LABRADOR, JP ;
BERGEMANN, AD ;
FLANAGAN, JG ;
PASQUALE, EB ;
KLEIN, R .
EMBO JOURNAL, 1995, 14 (13) :3116-3126
[8]  
BRANDLI AW, 1995, DEV DYNAM, V203, P119
[9]   LIGANDS FOR EPH-RELATED TYROSINE KINASE RECEPTORS ARE DEVELOPMENTALLY-REGULATED IN THE CNS [J].
CARPENTER, MK ;
SHILLING, H ;
VANDENBOS, T ;
BECKMANN, MP ;
CERRETTI, DP ;
KOTT, JN ;
WESTRUM, LE ;
DAVISON, BL ;
FLETCHER, FA .
JOURNAL OF NEUROSCIENCE RESEARCH, 1995, 42 (02) :199-206
[10]   IDENTIFICATION AND CLONING OF ELF-1, A DEVELOPMENTALLY EXPRESSED LIGAND FOR THE MEK4 AND SEK RECEPTOR TYROSINE KINASES [J].
CHENG, HJ ;
FLANAGAN, JG .
CELL, 1994, 79 (01) :157-168