GEMINI:: software for testing the effects of genotyping errors and multitubes approach for individual identification

被引：48

作者：

Valière, N

Berthier, P

Mouchiroud, D

Pontier, D

机构：

[1] Univ Lyon 1, UMR CNRS 5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, France

[2] Univ Bern, Inst Zool, CH-3012 Bern, Switzerland

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 01期

关键词：

allelic dropout; false allele; genotyping errors; individual identification; multitubes approach; software;

D O I：

10.1046/j.1471-8286.2002.00134.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Improvements in the determination of individual genotypes from samples with low DNA quantity and quality are of prime importance in molecular ecology and conservation for reliable genetic individual identification (molecular tagging using microsatllites loci). Thus, errors (e.g. allelic dropout and false allele) appearing during samples genotyping must be monitored and eliminated as far as possible. The multitubes approach is a very effective but a costly and time-consuming solution. In this paper, we present a simulation software that allows evaluation of the effect of genotyping errors on genetic identification of individuals and the effectiveness of a multitubes approach to correct these errors.

引用

页码：83 / 86

页数：4

共 18 条

[1] Noninvasive paternity assignment in Gombe chimpanzees [J].