Evaluation of the role of lactate dehydrogenase in oxalate synthesis

被引:14
作者
Sugiyama, N
Taniguchi, N
机构
[1] Faculty of Agriculture, University of Tokyo, Tokyo 113, Bunkyo-ku
关键词
Spinacia oleracea; Chenopodiaceae spinach; lactate dehydrogenase; oxalate;
D O I
10.1016/S0031-9422(96)00629-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The kinetic properties of lactate dehydrogenase (LDH) (EC 1.1.1.27) from spinach leaves were studied in order to evaluate the possible roles of LDH in the production of oxalate. LDH was purified by affinity chromatography on affigel blue and oxamate agarose columns. The pH optimum for the reduction of pyruvate was 7.25, while that for the reduction of glyoxylate was 7. The rate of reduction of pyruvate and glyoxylate at the optimum pH indicated substrate inhibition at concentrations above 8 and 40 mM, respectively. The maximum activity of LDH with pyruvate was about three times greater than with glyoxylate. The pH optimum for the oxidation of lactate was 9, with very low activity below pH 8. Substrate inhibition was apparent at lactate concentrations above 10 mM. LDH was inactive with glyoxylate in the oxidative reaction, which would lead to the biosynthesis of oxalate. Copyright (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:571 / 574
页数:4
相关论文
共 24 条
[1]  
ASKER H, 1984, PLANTA, V161, P272, DOI 10.1007/BF00982925
[2]   L-LACTATE DEHYDROGENASE FROM LEAVES OF CAPSELLA-BURSA-PASTORIS (L) MED .1. IDENTIFICATION AND PARTIAL CHARACTERIZATION [J].
BETSCHE, T ;
BOSBACH, K ;
GERHARDT, B .
PLANTA, 1979, 146 (05) :567-574
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   GLYOXYLATE INHIBITION OF RIBULOSEBISPHOSPHATE CARBOXYLASE OXYGENASE ACTIVATION IN INTACT, LYSED, AND RECONSTITUTED CHLOROPLASTS [J].
CAMPBELL, WJ ;
OGREN, WL .
PHOTOSYNTHESIS RESEARCH, 1990, 23 (03) :257-268
[6]   SYNTHESIS OF OXALIC-ACID BY ENZYMES FROM LETTUCE LEAVES [J].
DAVIES, DD ;
ASKER, H .
PLANT PHYSIOLOGY, 1983, 72 (01) :134-138
[7]   PURIFICATION AND PROPERTIES OF L(+)-LACTATE DEHYDROGENASE FROM POTATO-TUBERS [J].
DAVIES, DD ;
DAVIES, S .
BIOCHEMICAL JOURNAL, 1972, 129 (04) :831-&
[8]  
Davies DD, 1980, BIOCH PLANTS COMPREH, P581, DOI DOI 10.1016/B978-0-12-675402-5.50020-9
[9]   RATE OF OXALATE BIOSYNTHESIS FROM GLYCOLATE AND ASCORBIC-ACID IN SPINACH LEAVES [J].
FUJII, N ;
WATANABE, M ;
WATANABE, Y ;
SHIMADA, N .
SOIL SCIENCE AND PLANT NUTRITION, 1993, 39 (04) :627-634
[10]   THE ENZYMATIC REDUCTION OF GLYOXYLATE AND HYDROXYPYRUVATE IN LEAVES OF HIGHER-PLANTS [J].
GIVAN, CV ;
KLECZKOWSKI, LA .
PLANT PHYSIOLOGY, 1992, 100 (02) :552-556