High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance

被引：111

作者：

Bart, Rebecca ^{[1
]}

Cohn, Megan ^{[1
]}

Kassen, Andrew ^{[1
]}

McCallum, Emily J. ^{[2
]}

Shybut, Mikel ^{[1
]}

Petriello, Annalise ^{[1
]}

Krasileva, Ksenia ^{[1
]}

Dahlbeck, Douglas ^{[1
]}

Medina, Cesar ^{[3
]}

Alicai, Titus ^{[4
]}

Kumar, Lava ^{[5
]}

Moreira, Leandro M. ^{[6
]}

Rodrigues Neto, Jlio ^{[7
]}

Verdier, Valerie ^{[8
]}

Angelica Santana, Maria ^{[9
]}

Kositcharoenkul, Nuttima ^{[10
]}

Vanderschuren, Herve ^{[2
]}

Gruissem, Wilhelm ^{[2
]}

Bernal, Adriana ^{[3
]}

Staskawicz, Brian J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[2] ETH, Dept Biol, CH-8092 Zurich, Switzerland

[3] Univ Los Andes, Dept Biol Sci, Bogota, Colombia

[4] Natl Crops Resources Res Inst Namulonge, Kampala, Uganda

[5] Int Inst Trop Agr, Germplasm Hlth Unit, Ibadan, Nigeria

[6] Univ Fed Ouro Preto, Dept Ciencias Biol, Nucleo Pesquisas Ciencias Biol, Ouro Preto, MG, Brazil

[7] Ctr Expt Cent, Inst Biol, Inst Biol Secao Bacteriol Fitopatol, Lab Bacteriol Vegetal, BR-13012970 Campinas, SP, Brazil

[8] Univ Montpellier 2, Unite Mixte Rech, F-34394 Montpellier 5, France

[9] Univ Simon Bolivar, Dept Biol Celular, Caracas 1080, Venezuela

[10] Dept Agr, Plant Protect Res & Dev Off, Bangkok 10900, Thailand

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 28期

基金：

美国国家科学基金会; 美国食品与农业研究所;

关键词：

innate immunity; type three effectors; next-generation sequencing; AXONOPODIS PV. MANIHOTIS; PLANT INNATE IMMUNITY; PSEUDOMONAS-SYRINGAE; III EFFECTORS; TAL EFFECTORS; PTHA HOMOLOG; XANTHOMONAS; HOST; PATHOGENICITY; CAMPESTRIS;

D O I：

10.1073/pnas.1208003109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies.

引用

页码：E1972 / E1979

页数：8

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