Design of a luminescent biochip with nanodiamonds and bacterial luciferase

被引:23
作者
Puzyr', AP [1 ]
Pozdnyakova, IO [1 ]
Bondar', VS [1 ]
机构
[1] Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
关键词
Oxide; Enzyme; Aluminum; Spectroscopy; State Physics;
D O I
10.1134/1.1711469
中图分类号
O469 [凝聚态物理学];
学科分类号
070205 ;
摘要
An "aluminum oxide film-adhesive layer-nanodiamond-luciferase" supramolecular structure is prepared on a flat plate. It is demonstrated that, in this structure, the enzyme retains the catalytic activity. The structure prepared can be treated as a luminescent biochip prototype for use in bioluminescent analysis. (C) 2004 MAIK "Nauka / Interperiodica".
引用
收藏
页码:761 / 763
页数:3
相关论文
共 10 条
[1]  
[Anonymous], 1987, USP MIKROBIOL
[2]   MEASUREMENT OF CA-2+ CONCENTRATIONS IN LIVING CELLS [J].
BLINKS, JR ;
WIER, WG ;
HESS, P ;
PRENDERGAST, FG .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1982, 40 (1-2) :1-114
[3]  
BONDAR VS, 1988, PRIKL BIOKHIM MIKRO, V24, P745
[4]  
BONDAR' V S, 1991, Biokhimiya, V56, P806
[5]  
CHIGOT JP, 1981, ANN CHIR, V35, P5
[6]  
GITELZON II, 1984, LUMINOUS BACTERIA
[7]   TOTAL QUANTUM FLUX OF ISOTROPIC SOURCES [J].
HASTINGS, JW ;
WEBER, G .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA, 1963, 53 (12) :1410-&
[8]  
Illarionov BA, 2000, METHOD ENZYMOL, V305, P223
[9]  
PETUSHKOV V N, 1984, Biokhimiya, V49, P692
[10]  
Purtov K.V., 2001, DOKL AKAD NAUK, V380, P411