Osteogenetic Properties of Electrospun Nanofibrous PCL Scaffolds Equipped With Chitosan-Based Nanoreservoirs of Growth Factors

被引：65

作者：

Ferrand, Alice ^{[1
,2
]}

Eap, Sandy ^{[3
]}

Richert, Ludovic ^{[4
]}

Lemoine, Stephanie ^{[1
,3
]}

Kalaskar, Deepak ^{[5
]}

Demoustier-Champagne, Sophie ^{[5
]}

Atmani, Hassan ^{[7
]}

Mely, Yves ^{[4
]}

Fioretti, Florence ^{[1
,3
]}

Schlatter, Guy ^{[2
]}

Kuhn, Liisa ^{[6
]}

Ladam, Guy ^{[7
]}

Benkirane-Jessel, Nadia ^{[1
,8
]}

机构：

[1] INSERM, Osteoarticular & Dent Regenerat Nanomed UMR 1109, F-67085 Strasbourg, France

[2] Univ Strasbourg, CNRS,ICPEES UMR 7515, Inst Chim & Procedes Energie Environm & Sante, Inst Carnot MICA,Ecole Europeenne Chim Polymeres, F-67087 Strasbourg 2, France

[3] Univ Strasbourg, Fac Dent, F-67000 Strasbourg, France

[4] Fac Pharm, CNRS, UMR 7213, Biophoton & Pharmacol Lab, F-67401 Illkirch Graffenstaden, France

[5] Catholic Univ Leuven UCL, Inst Condensed Matter & Nanosci IMCN, Bio & Soft Matter BSMA, B-1348 Louvaine La Neuve la Neuve, Belgium

[6] Univ Connecticut, Dept Reconstruct Sci, Ctr Hlth, Farmington, CT 06030 USA

[7] Univ Rouen, Biophys & Biomat Lab La2B, EA MERCI 3829, Ctr Univ Evreux, F-27002 Evreux, France

[8] Univ Hosp Nancy, CNRS UMR 7561, Dept Orthopaed Surg, F-54035 Nancy, France

来源：

MACROMOLECULAR BIOSCIENCE | 2014年 / 14卷 / 01期

关键词：

biomimetic; bone morphogenetic protein; bone regeneration; layer-by-layer; poly(E-caprolactone); BONE MORPHOGENETIC PROTEIN-2; POLYELECTROLYTE MULTILAYERS; OCTACALCIUM PHOSPHATE; SERUM-ALBUMIN; TISSUE; DELIVERY; BIOMATERIALS; CHALLENGES; COLLAGEN; BIOLOGY;

D O I：

10.1002/mabi.201300283

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Bioactive implants intended for rapid, robust, and durable bone tissue regeneration are presented. The implants are based on nanofibrous 3D-scaffolds of bioresorbable poly-E-caprolactone mimicking the fibrillar architecture of bone matrix. Layer-by-layer nanoimmobilization of the growth factor BMP-2 in association with chitosan (CHI) or poly-L-lysine over the nanofibers is described. The osteogenetic potential of the scaffolds coated with layers of CHI and BMP-2 is demonstrated in vitro, and in vivo in mouse calvaria, through enhanced osteopontin gene expression and calcium phosphate biomineralization. The therapeutic strategy described here contributes to the field of regenerative medicine, as it proposes a route toward efficient repair of bone defects at reduced risk and cost level.

引用

页码：45 / 55

页数：11

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