LeFRK2, the gene encoding the major fructokinase in tomato fruits, is not required for starch biosynthesis in developing fruits

被引：34

作者：

Dai, N ^{[1
]}

German, MA ^{[1
]}

Matsevitz, T ^{[1
]}

Hanael, R ^{[1
]}

Swartzberg, D ^{[1
]}

Yeselson, Y ^{[1
]}

Petreikov, M ^{[1
]}

Schaffer, AA ^{[1
]}

Granot, D ^{[1
]}

机构：

[1] Agr Res Org, Volcani Ctr, Inst Field & Garden Crops, IL-50250 Bet Dagan, Israel

来源：

PLANT SCIENCE | 2002年 / 162卷 / 03期

关键词：

fructose; glucose; hexose phosphorylation; sucrose-synthase (SuSy); antisense; co-suppression;

D O I：

10.1016/S0168-9452(01)00583-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The LeFRK2 gene product has been proposed to play a role in transient starch accumulation in developing tomato (Lycopersicon esculentum) fruits. To analyze the role of LeFRK2 in starch biosynthesis, we produced transgenic tomato plants with sense and antisense expression of StFRK, the potato homologue to LeFRK2. Fruits of homozygous plants expressing sense or antisense StFRK exhibited suppression of LeFRK2, concomitantly with specific elimination of the FKI isozyme, indicating that FKI is the gene product of LeFRK2. The activity of fructokinase was reduced in antisense and sense homozygous fruits and increased in fruits of sense hemizygous plants. The modified activities of fructokinase led to small but significant changes both in the steady state levels of sugars and in the level of phosphorylated sugars in fruits. However, fruits lacking FKI had increased rather than decreased starch content. We therefore concluded that LeFRK2 is not required for transient starch accumulation in tomato fruits. Rather, LeFRK2 might have a role in the regulation of sucrose import into tomato fruits. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：423 / 430

页数：8

共 25 条

[1]

[Anonymous], 1991, PLANT TISSUE CULT

[2] The TYLCV-tolerant tomato line MP-1 is characterized by superior transformation competence [J].

Barg, R ;

Pilowsky, M ;

Shabtai, S ;

Carmi, N ;

Szechtman, AD ;

Dedicova, B ;

Salts, Y .

JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 (316) :1919-1923

[3] Transgenic tomato plants with decreased sucrose synthase are unaltered in starch and sugar accumulation in the fruit [J].

Chengappa, S ;

Guilleroux, M ;

Phillips, W ;

Shields, R .

PLANT MOLECULAR BIOLOGY, 1999, 40 (02) :213-221

[4] Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit [J].

D'Aoust, MA ;

Yelle, S ;

Nguyen-Quoc, B .

PLANT CELL, 1999, 11 (12) :2407-2418

[5] Potato (Solanum tuberosum L.) fructokinase expressed in yeast exhibits inhibition by fructose of both in vitro enzyme activity and rate of cell proliferation [J].

Dai, N ;

Schaffer, A ;

Petreikov, M ;

Granot, D .

PLANT SCIENCE, 1997, 128 (02) :191-197

[6]

Ho L.-C., 1986, The Tomato Crop, P201, DOI DOI 10.1007/978-94-009-3137-4_5

[7] Tomato fructokinases exhibit differential expression and substrate regulation [J].

Kanayama, Y ;

Granot, D ;

Dai, N ;

Petreikov, M ;

Schaffer, A ;

Powell, A ;

Bennett, AB .

PLANT PHYSIOLOGY, 1998, 117 (01) :85-90

[8] Divergent fructokinase genes are differentially expressed in tomato [J].

Kanayama, Y ;

Dai, N ;

Granot, D ;

Petreikov, M ;

Schaffer, A ;

Bennett, AB .

PLANT PHYSIOLOGY, 1997, 113 (04) :1379-1384

[9] Fgr, a major locus that modulates the fructose to glucose ratio in mature tomato fruits [J].

Levin, I ;

Gilboa, N ;

Yeselson, E ;

Shen, S ;

Schaffer, AA .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (02) :256-262

[10]

MartinezBarajas E, 1996, PLANTA, V199, P451, DOI 10.1007/BF00195739

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