Reversed-phase affinity chromatography of ecdysteroids with boronic acid-containing eluents

被引：1

作者：

Gildengorn, VD ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,KOMI SCI CTR,INST BIOL,SYKTYVKAR 167610,RUSSIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 730卷 / 1-2期

关键词：

mobile phase composition; ecdysteroids; boronic acid;

D O I：

10.1016/0021-9673(95)01094-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Selective effects of boronic acid-containing eluents on the chromatographic behaviour of ecdysteroids are described. The use of such eluents provides a simple, rapid and sensitive means to assess the presence of certain diol groups in ecdysteroids by ordinary RP-HPLC. It is demonstrated that ecdysteroids with different sets of diols in their molecules have individual shapes of chromatographic peaks. A mechanism of the boronic acid effect is discussed.

引用

页码：147 / 152

页数：6

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