TNF-alpha stimulates the biosynthesis of complement C3 and factor B by human umbilical cord vein endothelial cells

被引：27

作者：

Kawakami, Y

Watanabe, Y

Yamaguchi, M

Sakaguchi, H

Kono, I

Ueki, A

机构：

[1] KAWASAKI MED SCH, DEPT HYG, KURASHIKI, OKAYAMA 70101, JAPAN

[2] KAWASAKI MED SCH, DEPT GYNECOL, KURASHIKI, OKAYAMA 70101, JAPAN

来源：

CANCER LETTERS | 1997年 / 116卷 / 01期

关键词：

endothelial cells; complement; cytokines;

D O I：

10.1016/S0304-3835(97)04737-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The human umbilical cord vein (HUVEC) endothelial cells synthesize and secrete complement components. We analyzed the regulation of biosynthesis of the third component of complement (C3) and factor B (Bf) by cytokines in endothelial cells. Production of C3 increased after stimulation with TNF-alpha or IL-1 beta and that of Bf with TNF-alpha, TNF-beta and IFN-gamma. TNF-alpha was the most effective in stimulating the secretion of C3 and Bf or the expression of mRNA of C3 or Bf. Preincubation with TNF-alpha for at least 36 or 24 h was needed for the stimulation, respectively. TNF-alpha, together with IFN-gamma had synergistic effects and the release of C3 or Bf increased to about 40- and 26-fold higher than those in control cells after incubation with both cytokines. Published by Elsevier Science Ireland Ltd.

引用

页码：21 / 26

页数：6

共 23 条

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