Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria

被引：68

作者：

Molina-Navarro, MM ^{[1
]}

Casas, C ^{[1
]}

Piedrafita, L ^{[1
]}

Belli, G ^{[1
]}

Herrero, E ^{[1
]}

机构：

[1] Univ Lleida, Fac Med, Dept Ciencies Med Bas, Lleida 25008, Spain

来源：

FEBS LETTERS | 2006年 / 580卷 / 09期

关键词：

glutaredoxin; iron-sulfur cluster; mitochondria; redox regulation; glutathione;

D O I：

10.1016/j.febslet.2006.03.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The Saccharomyces cerevisiae monothiol glutaredoxin Grx5 participates in the mitochondrial biogenesis of iron-sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans. Chicken (cGRX5) and human (hGRX5) homologues contain a mitochondrial targeting sequence, suggesting a mitochondrial localization for these two proteins. We have compartmentalized the Escherichia coli and Synechocystis sp. homologues, and also cGRX5 and hGRX5, in the mitochondrial matrix of a yeast grx5 mutant. All four heterologous proteins rescue the defects of the mutant. The chicken cGRX5 gene was significantly expressed throughout the embryo stages in different tissues. These results underline the functional conservation of Grx5 homologues throughout evolution. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2273 / 2280

页数：8

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