Characterization of Bordetella strains and related bacteria by amplified ribosomal DNA restriction analysis and randomly and repetitive element-primed PCR

被引：4

作者：

Vandamme, P ^{[1
]}

Heyndrickx, M ^{[1
]}

DeRoose, I ^{[1
]}

Lammens, C ^{[1
]}

DeVos, P ^{[1
]}

Kersters, K ^{[1
]}

机构：

[1] UNIV INSTELLING ANTWERP HOSP,DEPT MED MICROBIOL,ANTWERP,BELGIUM

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1997年 / 47卷 / 03期

关键词：

D O I：

10.1099/00207713-47-3-802

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Amplified ribosomal DNA restriction analysis (ARDRA) in which the almost complete 16S rRNA gene was used as the target of the PCR assay and randomly and repetitive element-primed PCR were used to characterize 67 strains belonging to the family Alcaligenaceae. Particular emphasis was placed on strains of Bordetella pertussis (13 strains), Bordetella parapertussis (10 strains), and Bordetella bronchiseptica (10 strains) (these organisms were referred to as the B. bronchiseptica group), as well as Bordetella avium (19 strains), Our data indicate that strains belonging to the B. bronchiseptica group behave as a single species comparable to strains of B. avium. ARDRA allowed us to differentiate among all other species as well but was not useful for infraspecific typing. Randomly and repetitive element-primed PCR could be used to distinguish several infraspecific types within B. avium and the B. bronchiseptica group and allowed us to differentiate these two taxa.

引用

页码：802 / 807

页数：6

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