Effects of transforming growth factor β1 (TGFβ-1) and dentin non-collagenous proteins (DNCP) on human embryonic ectomesenchymal cells in a three-dimensional culture system

被引:28
作者
Deng, MJ
Shi, J
Smith, AJ
Jin, Y [1 ]
机构
[1] Fourth Mil Med Univ, Res & Dev Ctr Tissue Engn, Xian 710032, Shaanxi, Peoples R China
[2] Third Mil Med Univ, Daping Hosp, Dept Stomatol, Chongqing 400042, Peoples R China
[3] Fourth Mil Med Univ, Coll Stomatol, Dept Oral Histol & Pathol, Xian 710032, Shaanxi, Peoples R China
[4] Univ Birmingham, Sch Dent, Birmingham B4 6NN, W Midlands, England
关键词
ectomesenchymal cell; odontoblast; transforming growth factor beta 1; dentin non-collagenous protein; differentiation;
D O I
10.1016/j.archoralbio.2005.03.005
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Cranial neural crest-derived ectomesenchymal cells represent a population of pluripotent stem cells giving rise to many of the various oro-facial and dental tissues. The factors determining the terminal fate of these cells are still unclear. The potentiality of human embryonic ectomesenchymal cells from the first branchial arch have been investigated when isolated and grown in a three-dimensional (3D)-collagen get culture system in the presence of dentin matrix-derived non-collagenous proteins (DNCP) and TGF beta-1. Functional differentiation of cells showing some characteristics of odontobtast- like cells could be observed when the cells were cultured with DNCP+TGF beta-1 or DNCP, however, only cytological differentiation was observed during culture with TGF beta-1 alone. The characteristics of these cells was assessed by morphological appearance, expression of the odontoblast phenotype marker dentin sialophosphoprotein (DSPP), increased alkaline phosphatase levels and formation of mineralised nodules in vitro. The results indicate that these embryonic cells from the first branchial arch are capable of responding to the inductive stimulus of DNCP or DNCP + TGF beta-1 when isolated and grown in the 3D cotlagen get culture system. The capacity of the isolated cells to differentiate into mineralising cells showing some characteristics of odontoblast- like cells under these growth conditions highlights the potential of such approaches for tissue engineering strategies for hard-tissue regeneration after injury. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:937 / 945
页数:9
相关论文
共 39 条
[1]   Human dentin production in vitro [J].
About, I ;
Bottero, MJ ;
de Denato, P ;
Camps, J ;
Franquin, JC ;
Mitsiadis, TA .
EXPERIMENTAL CELL RESEARCH, 2000, 258 (01) :33-41
[2]   COMPARISON OF GROWTH AND CELL-PROLIFERATION KINETICS DURING MOUSE MOLAR ODONTOGENESIS INVIVO AND INVITRO [J].
AHMAD, N ;
RUCH, JV .
CELL AND TISSUE KINETICS, 1987, 20 (03) :319-329
[3]   Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats - similarities to astrocyte grafts [J].
Azizi, SA ;
Stokes, D ;
Augelli, BJ ;
DiGirolamo, C ;
Prockop, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3908-3913
[4]   Comparative analysis of mouse DSP and DPP expression in odontoblasts, preameloblasts, and experimentally induced odontoblast-like cells [J].
Bègue-Kirn, C ;
Ruch, JV ;
Ridall, BL ;
Butler, WT .
EUROPEAN JOURNAL OF ORAL SCIENCES, 1998, 106 :254-259
[5]  
BEGUEKIRN C, 1994, INT J DEV BIOL, V38, P405
[6]  
BEGUEKIRN C, 1992, INT J DEV BIOL, V36, P491
[7]   Bone marrow stromal stem cells: Nature, biology, and potential applications [J].
Bianco, P ;
Riminucci, M ;
Gronthos, S ;
Robey, PG .
STEM CELLS, 2001, 19 (03) :180-192
[8]   Extracellular matrix proteins and the dynamics of dentin formation [J].
Butler, WT ;
Brunn, JC ;
Qin, C ;
McKee, MD .
CONNECTIVE TISSUE RESEARCH, 2002, 43 (2-3) :301-307
[9]   Dentin matrix proteins [J].
Butler, WT .
EUROPEAN JOURNAL OF ORAL SCIENCES, 1998, 106 :204-210
[10]  
Cotton W.R., 1968, BIOL DENT PULP ORGAN, P69