Bisphenol A concentration-dependently increases human granulosa-lutein cell matrix metalloproteinase-9 (MMP-9) enzyme output

被引:47
作者
Dominguez, Miguel A. [1 ,2 ]
Petre, Maria A. [1 ]
Neal, Michael S. [1 ]
Foster, Warren G. [1 ]
机构
[1] McMaster Univ, Dept Obstet & Gynecol, Hamilton, ON L8N 3Z5, Canada
[2] Univ Autonoma Tamaulipas, Fac Med Vet & Zootecnia, Victoria, Tamaulipas, Mexico
基金
加拿大自然科学与工程研究理事会;
关键词
bisphenol A; granulosa cells; matrix metalloproteinases; MMP-9; folliculogenesis;
D O I
10.1016/j.reprotox.2008.05.059
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bisphenol A (BPA) is an estrogenic contaminant that has been quantified at higher levels in the follicular fluid of women with polycystic ovarian syndrome (PCOS) compared to healthy fertile controls. However, the effect of BPA on granulosa cell function is unknown. Therefore, the objective of the present study was to quantify the effect of BPA on granulosa cell progesterone (P4) output and matrix metalloproteinase (MMP)-2, and -9 output and activity. Granulosa-lutein cells (GLCs) were collected from women undergoing oocyte retrieval in an academic in vitro fertilization (IVF) program. Granulosa-lutein cells were treated with increasing log concentrations of BPA (1-10,000 ng/ml) or 17 beta-estradiol (E-2, 272 pg/ml or 1.0nM) and treatment effects on MMP-2 and -9 activity and output, cell viability and cell proliferation were measured by commercial gelatin zymography, MMP-ELISA, MTS and BrdU incorporation assays, respectively. Granulosa-lutein cells in culture secrete MMP-2 and MMP-9. Bisphenol A treatment concentration-dependently increased MMP-9 output by GLCs with a maximal effect observed at 1000 ng/ml. Cell viability/proliferation was unaffected by BPA treatment at concentrations <= 100 ng/ml; however, higher concentrations of BPA were cytotoxic. Progesterone Output by the GLCs was unaffected by increasing BPA concentrations in the media. In conclusion, GLCs in culture secrete MMP-2 and MMP-9. At lower concentrations, compatible with human exposure levels (100-1000 ng/ml), BPA stimulates GLC MMP-9 output; however, higher concentrations are cytotoxic. Our findings suggest that BPA treatment can modulate ovarian extracellular matrix stability. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:420 / 425
页数:6
相关论文
共 39 条
[1]   Hormonal characteristics of follicular fluid from women receiving either GnRH agonist or hCG for ovulation induction [J].
Andersen, C. Yding ;
Humaidan, P. ;
Ejdrup, H. Bredkjaer ;
Bungum, L. ;
Grondahl, M. L. ;
Westergaard, L. G. .
HUMAN REPRODUCTION, 2006, 21 (08) :2126-2130
[2]   Estradiol and regulation of anti-Mullerian hormone, inhibin-A, and inhibin-B secretion: Analysis of small antral and preovulatory human follicles' fluid [J].
Andersen, Claus Yding ;
Byskov, Anne Grete .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2006, 91 (10) :4064-4069
[3]   Differential distribution of gelatinases and tissue inhibitor of metalloproteinase-1 in the rat ovary [J].
Bagavandoss, P .
JOURNAL OF ENDOCRINOLOGY, 1998, 158 (02) :221-228
[4]   The early luteal phase administration of estrogen and progesterone does not induce premature luteolysis in normo-ovulatory women [J].
Beckers, Nicole G. M. ;
Platteau, Peter ;
Eijkemans, Marinus J. ;
Macklon, Nicholas S. ;
de Jong, Frank H. ;
Devroey, Paul ;
Fauser, Bart C. J. M. .
EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2006, 155 (02) :355-363
[5]  
Calafat AM, 2005, ENVIRON HEALTH PERSP, V113, P391, DOI 10.1289/ehp.7534
[6]   An update of luteal phase support in stimulated IVF cycles [J].
Fatemi, H. M. ;
Popovic-Todorovic, B. ;
Papanikolaou, E. ;
Donoso, P. ;
Devroey, P. .
HUMAN REPRODUCTION UPDATE, 2007, 13 (06) :581-590
[7]   Addition of estradiol to progesterone for luteal supplementation in patients stimulated with GnRH antagonist/rFSH for IVF: a randomized controlled trial [J].
Fatemi, H. M. ;
Kolibianakis, E. M. ;
Camus, M. ;
Tournaye, H. ;
Donoso, P. ;
Papanikolaou, E. ;
Devroey, P. .
HUMAN REPRODUCTION, 2006, 21 (10) :2628-2632
[8]   Bisphenol-A and chlorinated derivatives in adipose tissue of women [J].
Fernandez, M. F. ;
Arrebola, J. P. ;
Taoufiki, J. ;
Navalon, A. ;
Ballesteros, O. ;
Pulgar, R. ;
Vilchez, J. L. ;
Olea, N. .
REPRODUCTIVE TOXICOLOGY, 2007, 24 (02) :259-264
[9]   The role of the matrix metalloproteinases in human endometrial and ovarian cycles [J].
Goldman, S ;
Shalev, E .
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 2003, 111 (02) :109-121
[10]   Weight of the evidence evaluation of low-dose reproductive and developmental effects of bisphenol A [J].
Gray, GM ;
Cohen, JT ;
Cunha, G ;
Hughes, C ;
McConnell, EE ;
Rhomberg, L ;
Sipes, IG ;
Mattison, D .
HUMAN AND ECOLOGICAL RISK ASSESSMENT, 2004, 10 (05) :875-921