Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models

被引：26

作者：

Larina, Irina V. ^{[1
]}

Syed, Saba H. ^{[1
]}

Sudheendran, Narendran ^{[3
]}

Overbeek, Paul A. ^{[2
]}

Dickinson, Mary E. ^{[1
]}

Larin, Kirill V. ^{[1
,3
]}

机构：

[1] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA

[3] Univ Houston, Dept Biomed Engn, Houston, TX 77204 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 08期

基金：

美国国家卫生研究院;

关键词：

optical coherence tomography; mammalian; mouse; embryonic imaging; eye development; in utero; FUNCTIONAL ANNOTATION; TRANSGENIC MICE; RETINOBLASTOMA; EYE;

D O I：

10.1117/1.JBO.17.8.081410

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse models of ocular diseases provide a powerful resource for exploration of molecular regulation of eye development and pre-clinical studies. Availability of a live high-resolution imaging method for mouse embryonic eyes would significantly enhance longitudinal analyses and high-throughput morphological screening. We demonstrate that optical coherence tomography (OCT) can be used for live embryonic ocular imaging throughout gestation. At all studied stages, the whole eye is within the imaging distance of the system and there is a good optical contrast between the structures. We also performed OCT eye imaging in the embryonic retinoblastoma mouse model Pax6-SV40 T-antigen, which spontaneously forms lens and retinal lesions, and demonstrate that OCT allows us to clearly differentiate between the mutant and wild type phenotypes. These results demonstrate that OCT in utero imaging is a potentially useful tool to study embryonic ocular diseases in mouse models. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.8.081410]

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