Intersimple sequence repeats (ISSR) molecular fingerprinting markers for authenticating populations of Dendrobium officinale Kimura et Migo

被引：48

作者：

Shen, J ^{[1
]}

Ding, XY ^{[1
]}

Liu, DY ^{[1
]}

Ding, G ^{[1
]}

He, J ^{[1
]}

Li, XX ^{[1
]}

Tang, F ^{[1
]}

Chu, BH ^{[1
]}

机构：

[1] Nanjing Normal Univ, Jiangsu Key Lab Biodivers & Biotechnol, Coll Life Sci, Nanjing 210097, Peoples R China

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2006年 / 29卷 / 03期

关键词：

intersimple sequence repeats; Dendrobium officinale; population; molecular authentication;

D O I：

10.1248/bpb.29.420

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Intersimple sequence repeats (ISSR) molecular fingerprinting markers have been employed to authenticate eight populations of Dendrobium officinale using 10 primers selected from 76 ISSR primers. A total of 127 DNA fragments were amplified, of which 115 were polymorphic (90.5% of all bands). Sixteen specific authentication markers have been found. To enhance the efficiency of authentication, ISSR fingerprinting codes have been constructed using six polymorphic bands for authenticating D. officinale populations. Eight wild populations of D. officinale have been authenticated accurately using ISSR.

引用

页码：420 / 422

页数：3

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