Variation in Gene Expression of Inflammatory Cytokines in Leukocyte-Derived Cells of High-Fat-Diet-Induced Insulin-Resistant Rats

被引：13

作者：

Fujimoto, Saki ^{[1
]}

Mochizuki, Kazuki ^{[1
]}

Shimada, Masaya ^{[1
]}

Murayama, Yuki ^{[1
]}

Goda, Toshinao ^{[1
]}

机构：

[1] Univ Shizuoka, Global COE Program, Grad Sch Nutr & Environm Sci, Lab Nutr Physiol, Shizuoka 4228526, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 10期

关键词：

high-fat diet; insulin resistance; interleukin-1; beta; leukocyte; tumor necrosis factor-alpha;

D O I：

10.1271/bbb.80259

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-fat diet is thought to enhance inflammation in various tissues by increasing insulin resistance. In this study, we determined the mRNA levels of inflammatory cytokines in leukocyte-derived cells in the blood of rats with high-fat-diet-induced insulin resistance. Feeding rats a high-fat diet for 77d induced moderate insulin resistance, which was determined by increased plasma glucose and insulin concentrations, following an oral glucose tolerance test. The interleukin (IL)-1 beta mRNA level was higher in the insulin-resistant rats than in control rats at the fasting stage, whereas the tumor necrosis factor (TNF)-alpha mRNA level was greatly elevated at 180 min after glucose administration in the insulin-resistant rats. The results suggest that feeding rats a high-fat diet enhances the expression of fasting IL-1 beta and postprandial TNF-alpha genes in leukocyte-derived cells.

引用

页码：2572 / 2579

页数：8

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