On-column gas chromatographic mass spectrometric assay for metabolic profiling of valproate in brain tissue and serum

被引：15

作者：

Darius, J

机构：

[1] Department of Clinical Pharmacology, University Hospital

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 682卷 / 01期

关键词：

derivatization; GC; valproate;

D O I：

10.1016/0378-4347(95)00559-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive capillary gas chromatographic-mass spectrometric method for the determination of valproic acid and at least twelve of its metabolites in serum based on tert.-butyldimethylsilyl (tBDMS) derivatives is described. Low detection limits are achieved by using a direct on-column injection technique. The addition of dry pyridine during the derivatization step now leads to uniform formation of 3-keto-VPA di-tBDMS derivatives and thereby avoids the necessity of a deuterated internal standard. A novel extraction procedure for metabolic profiling of valproate in brain tissue samples is presented. Using this method, (Z)-2-en-VPA was determined in rat brain tissue for the first time.

引用

页码：67 / 72

页数：6

共 27 条

[1] CAPILLARY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY OF VALPROIC ACID METABOLITES IN SERUM AND URINE USING TERT-BUTYLDIMETHYLSILYL DERIVATIVES
ABBOTT, FS
KASSAM, J
ACHEAMPONG, A
FERGUSON, S
PANESAR, S
BURTON, R
FARRELL, K
ORR, J
[J]. JOURNAL OF CHROMATOGRAPHY, 1986, 375 (02): : 285 - 298
[2] QUANTITATIVE STRUCTURE ANTICONVULSANT ACTIVITY RELATIONSHIPS OF VALPROIC ACID, RELATED CARBOXYLIC-ACIDS AND TETRAZOLES
ABBOTT, FS
ACHEAMPONG, AA
[J]. NEUROPHARMACOLOGY, 1988, 27 (03) : 287 - 294
[3] IDENTIFICATION OF VALPROIC ACID METABOLITES IN HUMAN-SERUM AND URINE USING HEXADEUTERATED VALPROIC ACID AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS
ACHEAMPONG, A
ABBOTT, F
BURTON, R
[J]. BIOMEDICAL MASS SPECTROMETRY, 1983, 10 (11): : 586 - 595
[4] DISTRIBUTION OF UNSATURATED METABOLITES OF VALPROATE IN HUMAN AND RAT-BRAIN - PHARMACOLOGICAL RELEVANCE
ADKISON, KDK
OJEMANN, GA
RAPPORT, RL
DILLS, RL
SHEN, DD
[J]. EPILEPSIA, 1995, 36 (08) : 772 - 782
[5] Baillie Thomas A., 1995, P589
[6] SENSITIVE CAPILLARY GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC METHOD FOR THE THERAPEUTIC DRUG-MONITORING OF VALPROIC ACID AND 7 OF ITS METABOLITES IN HUMAN SERUM - APPLICATION OF THE ASSAY FOR A GROUP OF PEDIATRIC EPILEPTICS
DARIUS, J
MEYER, FP
[J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 656 (02): : 343 - 351
[7] ANTICONVULSANT AND NEUROTOXIC ACTIVITIES OF 12 ANALOGS OF VALPROIC ACID
ELMAZAR, MMA
HAUCK, RS
NAU, H
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1993, 82 (12) : 1255 - 1258
[8] QUANTITATIVE-DETERMINATION OF VALPROIC ACID AND 14 METABOLITES IN SERUM AND URINE BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
FISHER, E
WITTFOHT, W
NAU, H
[J]. BIOMEDICAL CHROMATOGRAPHY, 1992, 6 (01) : 24 - 29
[9] GRANNEMAN GR, 1991, IDIOSYNCRATIC REACTI, P59
[10] METABOLIC PROFILING OF VALPROIC ACID IN PATIENTS USING NEGATIVE-ION CHEMICAL IONIZATION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
KASSAHUN, K
FARRELL, K
ZHENG, JJ
ABBOTT, F
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 527 (02): : 327 - 341

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