Identification of a variable number tandem repeat region in the human T cell receptor alpha-delta (TCRAD) locus

被引：4

作者：

Buchmayer, H

Rumpold, H

Mannhalter, C

机构：

[1] UNIV VIENNA, GEN HOSP VIENNA, DIV MOL BIOL, DEPT LAB MED, A-1090 VIENNA, AUSTRIA

[2] UNIV VIENNA, GEN HOSP VIENNA, DIV IMMUNOL, A-1090 VIENNA, AUSTRIA

来源：

HUMAN GENETICS | 1996年 / 98卷 / 03期

关键词：

D O I：

10.1007/s004390050218

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A number of different polymorphisms have been observed in coding as well as in non-coding regions of T cell receptor (TCR) genes. We report the identification and characterization of a highly polymorphic locus in the 3' noncoding region of the human T cell receptor alpha/delta (TCRAD) on chromosome 14. In 202 unrelated individuals, ten different alleles were distinguished by polymerase chain reaction (PCR) and a heterozygosity rate of 64% was calculated. Sequence analysis revealed that this polymorphic region consists of 10 bp imperfect repeat units and represents a variable number tandem repeat region (VNTR). Stable Mendelian inheritance of this novel polymorphic marker was proven in four families. The localization of this VNTR polymorphism in the TCRAD locus should make it a useful system for linkage analysis in immunological disorders with a known role of TCRAD.

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收藏

页码：333 / 335

页数：3

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