High inducibility of mouse renal CYP2E1 gene by tobacco smoke and its possible effect on DNA single strand breaks

被引:34
作者
Seree, EM
Villard, PH
Re, JL
DeMeo, M
Lacarelle, B
Attolini, L
Dumenil, G
Catalin, J
Durand, A
Barra, Y
机构
[1] FAC PHARM MARSEILLE,EA 859,LAB PHARMACOCINET & TOXICOL,F-13385 MARSEILLE 5,FRANCE
[2] FAC PHARM MARSEILLE,URA CNRS 1924,F-13385 MARSEILLE 5,FRANCE
[3] FAC MED,LAB PHARMACOL CLIN PR BRUGUEROLLE,F-13385 MARSEILLE 5,FRANCE
关键词
D O I
10.1006/bbrc.1996.0250
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several studies have shown in humans an association between renal carcinoma and cigarette smoking. Cigarette smoke contains numerous cytochrome P450 inducers and substrates. In the present study we investigated the effect of cigarette smoke on the regulation of murine cytochrome P450 expression in kidney and its possible role in the induction of single strand breaks of DNA. Results demonstrated that CYP2E1 (activity, protein, and mRNA) was induced by tobacco smoke (2.1, 5.6, and 20.8, respectively). We did not detect any CYP1A, CYP2B, and CYP3A using Western blot and RT-PCR experiments. We have analyzed the renal single strand breaks of DNA in control and treated mice. The results indicated an increase of single strand breaks of DNA in kidney from treated mice which paralleled the high inducibility of the CYP2E1. No significant difference was observed between lymphocytes (which expressed very low or undetectable cytochrome P450 levels) of control and treated mice. (C) 1996 Academic Press, Inc
引用
收藏
页码:429 / 434
页数:6
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