Characterization of the gene encoding murine heparin-binding epidermal growth factor-like growth factor

被引：11

作者：

Harding, PA

Brigstock, DR

Shen, LM

CrissmanCombs, MA

Besner, GE

机构：

[1] CHILDRENS HOSP,DEPT SURG,COLUMBUS,OH 43205

[2] OHIO STATE UNIV,DEPT SURG,COLUMBUS,OH 43205

[3] OHIO STATE UNIV,DEPT PEDIAT,COLUMBUS,OH 43205

来源：

GENE | 1996年 / 169卷 / 02期

关键词：

mHB-EGF; lambda phage; DNA sequencing; PCR; intron; splice donor and acceptor site;

D O I：

10.1016/0378-1119(95)00861-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

The mouse gene (mHB-EGF) encoding heparin-binding epidermal growth factor-like growth factor was isolated from a mouse 129SVJ genomic library. DNA sequence analysis confirmed that the clone contained six exons (I-VI) and five introns (A-E), and spanned approx. 14 kb of DNA. PCR analysis showed that introns A-E of mHB-EGF are 203 bp, 2.5 kb, 5.5 kb, 825 bp and 272 bp in length, respectively. These results establish that mHB-EGF is similar in organization to human HB-EGF (hHB-EGF). However, DNA sequence analysis of introns A-E of mHB-EGF failed to show significant overall homology with those of hHB-EGF.

引用

页码：291 / 292

页数：2

共 7 条

[1]

HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR - CHARACTERIZATION OF RAT AND MOUSE CDNA CLONES, PROTEIN DOMAIN CONSERVATION ACROSS SPECIES, AND TRANSCRIPT EXPRESSION IN TISSUES [J].