C5a, But Not C3a, Increases VEGF Secretion in ARPE-19 Human Retinal Pigment Epithelial Cells

被引：48

作者：

Cortright, Daniel N. ^{[1
]}

Meade, Robin ^{[1
]}

Waters, Stephen M. ^{[1
]}

Chenard, Bertrand L. ^{[1
]}

Krause, James E. ^{[1
]}

机构：

[1] Neurogen Corp, Branford, CT USA

来源：

CURRENT EYE RESEARCH | 2009年 / 34卷 / 01期

关键词：

ARPE-19; C3a; C5a; C5L2; VEGF; MACULAR DEGENERATION; RECEPTOR; PROTEIN; PREDISPOSES; DISEASE; BINDING; LINE;

D O I：

10.1080/02713680802546658

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

Purpose: We examined the potential for the pro-inflammatory complement proteins C5a and C3a to increase VEGF expression in ARPE-19 cells. Materials and Methods: Expression of complement receptors in ARPE-19 cells was evaluated by RT-PCR. VEGF secretion from ARPE-19 cells treated with C5a or C3a was determined by ELISA. Results: C5a and C3a receptor, but not C5L2, were detected in human eye tissue and ARPE-19 cells. C5a, but not C3a, treatment increased VEGF secretion from ARPE-19 cells, an effect inhibited by the C5aR antagonist, NDT 9513727. Conclusions: C5a receptor mediates increased VEGF secretion from ARPE-19 cells, suggesting a role for the C5a receptor in the pathogenesis of macular degeneration.

引用

页码：57 / 61

页数：5

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