Identification of glycoproteins on nitrocellulose membranes and gels

被引：48

作者：

Thornton, DJ ^{[1
]}

Carlstedt, I ^{[1
]}

Sheehan, JK ^{[1
]}

机构：

[1] UNIV MANCHESTER,SCH BIOL SCI,WELLCOME TRUST CTR CELL MATRIX RES,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

MOLECULAR BIOTECHNOLOGY | 1996年 / 5卷 / 02期

关键词：

mucins; glycoprotein; periodic acid Schiff; alcian blue; high-iron diamine; lectins;

D O I：

10.1007/BF02789065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this article we describe a procedure for the detection of glycoproteins on gels employing the periodic acid-Schiff's reagent. In addition, a number of staining protocols and direct binding ELISA, employing antibodies and lectins, are described for the identification and quantitation of glycoproteins after their immobilization by dot, slot, or Western blotting onto nitrocellulose membranes. We document, in detail, the conditions (i.e., the effect of solvent and detergents) for the immobilization of one specific family of O-linked glycoproteins, namely mucins. However, taking into account our suggestions, these procedures should be applicable to other types of glycoprotein.

引用

页码：171 / 176

页数：6

共 10 条

[1]

Carlstedt I, 1989, Symp Soc Exp Biol, V43, P289

[2] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].