Tobacco OPBP1 Enhances Salt Tolerance and Disease Resistance of Transgenic Rice

被引：48

作者：

Chen, Xujun ^{[1
]}

Guo, Zejian ^{[1
]}

机构：

[1] China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2008年 / 9卷 / 12期

基金：

高等学校博士学科点专项科研基金;

关键词：

AP2/EREBP; salt tolerance; disease resistance; transcription factor; transgenic rice;

D O I：

10.3390/ijms9122601

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osmotin promoter binding protein 1 ( OPBP1), an AP2/ERF transcription factor of tobacco, has been demonstrated to function in disease resistance and salt tolerance in tobacco. To increase stress tolerant capability of rice, we generated rice plants with an OPBP1 overexpressing construct. Salinity shock treatment with 250 mM NaCl indicated that most of the OPBP1 transgenic plants can survive, whereas the control seedlings cannot. Similar recovery was found by using the seedlings grown in 200 mM NaCl for two weeks. The OPBP1 transgenic and control plants were also studied for oxidative stress tolerance by treatment with paraquat, showing the transgenic lines were damaged less in comparison with the control plants. Further, the OPBP1 overexpression lines exhibited enhanced resistance to infections of Magnaporthe oryzae and Rhizoctonia solani pathogens. Gene expressing analysis showed increase in mRNA accumulation of several stress related genes. These results suggest that expression of OPBP1 gene increase the detoxification capability of rice.

引用

页码：2601 / 2613

页数：13

共 24 条

[1] ADAPTATIONS TO ENVIRONMENTAL STRESSES [J].

BOHNERT, HJ ;

NELSON, DE ;

JENSEN, RG .

PLANT CELL, 1995, 7 (07) :1099-1111

[2] Carotenoids .3. In vivo functions of carotenoids in higher plants [J].

DemmigAdams, B ;

Gilmore, AM ;

Adams, WW .

FASEB JOURNAL, 1996, 10 (04) :403-412

[3] OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression [J].

Dubouzet, JG ;

Sakuma, Y ;

Ito, Y ;

Kasuga, M ;

Dubouzet, EG ;

Miura, S ;

Seki, M ;

Shinozaki, K ;

Yamaguchi-Shinozaki, K .

PLANT JOURNAL, 2003, 33 (04) :751-763

[4] Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses [J].

Garg, AK ;

Kim, JK ;

Owens, TG ;

Ranwala, AP ;

Do Choi, Y ;

Kochian, LV ;

Wu, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :15898-15903

[5] Overexpression of the AP2/EREBP transcription factor OPBP1 enhances disease resistance and salt tolerance in tobacco [J].

Guo, ZJ ;

Chen, XJ ;

Wu, XL ;

Ling, JQ ;

Xu, P .

PLANT MOLECULAR BIOLOGY, 2004, 55 (04) :607-618

[6] Plant cellular and molecular responses to high salinity [J].

Hasegawa, PM ;

Bressan, RA ;

Zhu, JK ;

Bohnert, HJ .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2000, 51 :463-499

[7] EFFICIENT TRANSFORMATION OF RICE (ORYZA-SATIVA L) MEDIATED BY AGROBACTERIUM AND SEQUENCE-ANALYSIS OF THE BOUNDARIES OF THE T-DNA [J].

HIEI, Y ;

OHTA, S ;

KOMARI, T ;

KUMASHIRO, T .

PLANT JOURNAL, 1994, 6 (02) :271-282

[8] Characterization of the gene for Delta(1)-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L [J].

Igarashi, Y ;

Yoshiba, Y ;

Sanada, Y ;

YamaguchiShinozaki, K ;

Wada, K ;

Shinozaki, K .

PLANT MOLECULAR BIOLOGY, 1997, 33 (05) :857-865

[9] Rapid determination of rice cultivar responses to the sheath blight pathogen Rhizoctonia solani using a micro-chamber screening method [J].

Jia, Y. ;

Correa-Victoria, F. ;

McClung, A. ;

Zhu, L. ;

Liu, G. ;

Wamishe, Y. ;

Xie, J. ;

Marchetti, M. A. ;

Pinson, S. R. M. ;

Rutger, J. N. ;

Correll, J. C. .

PLANT DISEASE, 2007, 91 (05) :485-489

[10] The barley ERF-type transcription factor HvRAF confers enhanced pathogen resistance and salt tolerance in Arabidopsis [J].

Jung, Jinwook ;

Won, So Youn ;

Suh, Seok Cheol ;

Kim, HyeRan ;

Wing, Rod ;

Jeong, Yeonhwa ;

Hwang, Ingyu ;

Kim, Minkyun .

PLANTA, 2007, 225 (03) :575-588

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