Real-time PCR for characterizing the stress response of Oenococcus oeni in a wine-like medium

被引：68

作者：

Beltramo, C ^{[1
]}

Desroche, N ^{[1
]}

Tourdot-Maréchal, R ^{[1
]}

Grandvalet, C ^{[1
]}

Guzzo, J ^{[1
]}

机构：

[1] ENSBANA, INRA, UMR 1232, UB,Lab Microbiol, F-21000 Dijon, France

来源：

RESEARCH IN MICROBIOLOGY | 2006年 / 157卷 / 03期

关键词：

lactic acid bacteria; Oenococcus oeni; malolactic fermentation; real-time RT-PCR; stress; transcription; wine;

D O I：

10.1016/j.resmic.2005.07.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in wine. To optimize survival and growth in wine, cells were adapted during growth at low pH (3.5) prior to inoculation into wine. The level of gene expression was analyzed after growth at pH 3.5 in a rich medium and during MLF in a wine-like medium. RT-qPCR analyses exhibited different expression ratios of stress genes. The data obtained showed that determination of mRNA levels could constitute a new approach to studying the stress response of O. oeni after adaptation at low pH and during growth in a wine-like medium. (c) 2005 Elsevier SAS. All rights reserved.

引用

页码：267 / 274

页数：8

共 34 条

[1] Protease A activity and nitrogen fractions released during alcoholic fermentation and autolysis in enological conditions
Alexandre, H
Heintz, D
Chassagne, D
Guilloux-Benatier, M
Charpentier, C
Feuillat, M
[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2001, 26 (04) : 235 - 240
[2] Evidence for multiple levels of regulation of Oenococcus oeni clpP-clpL locus expression in response to stress
Beltramo, C
Grandvalet, C
Pierre, F
Guzzo, J
[J]. JOURNAL OF BACTERIOLOGY, 2004, 186 (07) : 2200 - 2205
[3] Carreté R, 2002, FEMS MICROBIOL LETT, V211, P155, DOI 10.1016/S0378-1097(02)00687-0
[4] MEDIUM FOR SCREENING LEUCONOSTOC-OENOS STRAINS DEFECTIVE IN MALOLACTIC FERMENTATION
CAVIN, JF
PREVOST, H
LIN, J
SCHMITT, P
DIVIES, C
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (03) : 751 - 753
[5] Real-time reverse transcription-PCR analysis of expression of halobenzoate and salicylate catabolism-associated operons in two strains of Pseudomonas aeruginosa
Corbella, ME
Puyet, A
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) : 2269 - 2275
[6] Membrane fluidity adjustments in ethanol-stressed Oenococcus oeni cells
Da Silveira, MG
Golovina, EA
Hoekstra, FA
Rombouts, FM
Abee, T
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (10) : 5826 - 5832
[7] Flow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cells
da Silveira, MG
San Romao, MV
Loureiro-Dias, MC
Rombouts, FM
Abee, T
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) : 6087 - 6093
[8] CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in Gram-positive bacteria
Derré, I
Rapoport, G
Msadek, T
[J]. MOLECULAR MICROBIOLOGY, 1999, 31 (01) : 117 - 131
[9] Determination of an internal control to apply reverse transcription quantitative PCR to study stress response in the lactic acid bacterium Oenococcus oeni
Desroche, N
Beltramo, C
Guzzo, J
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 60 (03) : 325 - 333
[10] Real-time reverse transcription PCR analysis of expression of atrazine catabolism genes in two bacterial strains isolated from soil
Devers, M
Soulas, G
Martin-Laurent, F
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 56 (01) : 3 - 15

← 1 2 3 4 →