Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

被引:93
作者
Chan, Ming-Chang [1 ]
Ho, Shih-Hsin [1 ]
Lee, Duu-Jong [2 ]
Chen, Chun-Yen [3 ]
Huang, Chieh-Chen [4 ]
Chang, Jo-Shu [1 ,3 ,4 ,5 ,6 ]
机构
[1] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan
[2] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei, Taiwan
[3] Natl Cheng Kung Univ, Ctr Biosci & Biotechnol, Tainan 70101, Taiwan
[4] Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung 40227, Taiwan
[5] Natl Cheng Kung Univ, Res Ctr Energy Technol, Tainan 70101, Taiwan
[6] Natl Cheng Kung Univ, Strategy Ctr, Tainan 70101, Taiwan
关键词
Microalgae; Scenedesmus obliquus; Lutein; Extraction kinetics; Bioseparations; Purification; BETA-CAROTENE; BIOMASS; PHOTOSYNTHESIS; DECOLORIZATION; SAPONIFICATION; ACCUMULATION; ANTIOXIDANTS; ASTAXANTHIN; CHLOROPHYTA; ALMERIENSIS;
D O I
10.1016/j.bej.2012.11.017
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 090105 [作物生产系统与生态工程];
摘要
This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24 h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20 degrees C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:24 / 31
页数:8
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