Challenges in applying microarrays to environmental studies

被引：128

作者：

Zhou, JZ ^{[1
]}

Thompson, DK ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2002年 / 13卷 / 03期

关键词：

D O I：

10.1016/S0958-1669(02)00319-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although DNA microarray technology has been used successfully to analyze global gene expression in pure cultures, it has not been rigorously tested and evaluated within the context of complex environmental samples. Adapting microarray hybridization for use in environmental studies faces several challenges associated with specificity, sensitivity and quantitation.

引用

页码：204 / 207

页数：4

共 24 条

[1] Portable system for microbial sample preparation and oligonucleotide microarray analysis [J].

Bavykin, SG ;

Akowski, JP ;

Zakhariev, VM ;

Barsky, VE ;

Perov, AN ;

Mirzabekov, AD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (02) :922-928

[2] Accessing genetic information with high-density DNA arrays [J].

Chee, M ;

Yang, R ;

Hubbell, E ;

Berno, A ;

Huang, XC ;

Stern, D ;

Winkler, J ;

Lockhart, DJ ;

Morris, MS ;

Fodor, SPA .

SCIENCE, 1996, 274 (5287) :610-614

[3] Bacterial species determination from DNA-DNA hybridization by using genome fragments and DNA microarrays [J].

Cho, JC ;

Tiedje, JM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (08) :3677-3682

[4] Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays [J].

de Saizieu, A ;

Certa, U ;

Warrington, J ;

Gray, C ;

Keck, W ;

Mous, J .

NATURE BIOTECHNOLOGY, 1998, 16 (01) :45-48

[5] Exploring the metabolic and genetic control of gene expression on a genomic scale [J].

DeRisi, JL ;

Iyer, VR ;

Brown, PO .

SCIENCE, 1997, 278 (5338) :680-686

[6] Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology [J].

Guschin, DY ;

Mobarry, BK ;

Proudnikov, D ;

Stahl, DA ;

Rittmann, BE ;

Mirzabekov, AD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (06) :2397-2402

[7] DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli [J].

Khodursky, AB ;

Peter, BJ ;

Cozzarelli, NR ;

Botstein, D ;

Brown, PO ;

Yanofsky, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :12170-12175

[8] Expression monitoring by hybridization to high-density oligonucleotide arrays [J].

Lockhart, DJ ;

Dong, HL ;

Byrne, MC ;

Follettie, MT ;

Gallo, MV ;

Chee, MS ;

Mittmann, M ;

Wang, CW ;

Kobayashi, M ;

Horton, H ;

Brown, EL .

NATURE BIOTECHNOLOGY, 1996, 14 (13) :1675-1680

[9] High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays [J].

Pinkel, D ;

Seagraves, R ;

Sudar, D ;

Clark, S ;

Poole, I ;

Kowbel, D ;

Collins, C ;

Kuo, WL ;

Chen, C ;

Zhai, Y ;

Dairkee, SH ;

Ljung, BM ;

Gray, JW ;

Albertson, DG .

NATURE GENETICS, 1998, 20 (02) :207-211

[10] Genome-wide analysis of DNA copy-number changes using cDNA microarrays [J].

Pollack, JR ;

Perou, CM ;

Alizadeh, AA ;

Eisen, MB ;

Pergamenschikov, A ;

Williams, CF ;

Jeffrey, SS ;

Botstein, D ;

Brown, PO .

NATURE GENETICS, 1999, 23 (01) :41-46

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