Vaccination strategies to enhance local immunity and protection against Mycobacterium tuberculosis

被引：13

作者：

Klucar, Peter ^{[1
]}

Barnes, Peter F. ^{[1
,2
]}

Kong, Ying ^{[1
]}

Howard, Susan T. ^{[1
,2
]}

Pang, Xiuhua ^{[1
]}

Huang, Fang-Fang ^{[1
]}

Tvinnereim, Amy R. ^{[1
]}

Samten, Buka ^{[1
,2
]}

Shams, Homayoun ^{[1
,2
]}

机构：

[1] Univ Texas Hlth Sci Ctr Tyler, Ctr Pulm & Infect Dis Control, Tyler, TX 75708 USA

[2] Univ Texas Hlth Sci Ctr Tyler, Dept Microbiol & Immunol, Tyler, TX 75708 USA

来源：

VACCINE | 2009年 / 27卷 / 12期

关键词：

CFP10; Tuberculosis; Local immunity; CD8(+) T-CELLS; GENETIC IMMUNIZATION; MUCOSAL IMMUNITY; DNA IMMUNIZATION; PLASMID DNA; BOVIS BCG; IFN-GAMMA; IN-VIVO; INFECTION; CD4(+);

D O I：

10.1016/j.vaccine.2009.01.119

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57BlI/6 DR4 mice that were transgenic for human HLA DRB1 *0401, because CFP10 contains epitopes for DRB1 *0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFPIO elicited production of IFN-gamma by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-gamma production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis. (C) 2009 Elsevier Ltd. All rights reserved

引用

页码：1816 / 1824

页数：9

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