On the structure of endogenous ouabain

被引:170
作者
Kawamura, A
Guo, JS
Itagaki, Y
Bell, C
Wang, Y
Haupert, GT
Magil, S
Gallagher, RT
Berova, N
Nakanishi, K [1 ]
机构
[1] Columbia Univ, Dept Chem, New York, NY 10027 USA
[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Med Serv,Renal Unit, Charlestown, MA 02129 USA
[3] BION Inc, Riverside Technol Ctr, Cambridge, MA 02139 USA
关键词
D O I
10.1073/pnas.96.12.6654
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The ouabain-like sodium pump inhibitor in mammals (so-called "endogenous ouabain") has been considered a subtle structural isomer of ouabain. Its structural investigation, however, has long been hindered by the paucity of sample material, Our recent purification of endogenous ouabain (3 mu g) from bovine hypothalamus allowed the measurement of its H-1-NMR, The obtained spectrum as well as reexamination of past microscale structural studies on endogenous ouabain led us to identify the purified material as ouabain in an unusual manner. It turned out that the structural analysis had been complicated by a facile ouabain-borate complexation in borosilicate glassware. In retrospect, it is not surprising that the polyhydroxylated ouabain molecule serves as a polydentate ligand to inorganic species. In its physiological environment, ouabain may exist as some unknown complex, The chemical species giving rise to the reported biological activities of hypothalamic inhibitory factor preparations remain to be clarified.
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页码:6654 / 6659
页数:6
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