Specific downregulation of bcl-2 and xIAP by RNAi enhances the effects of chemotherapeutic agents in MCF-7 human breast cancer cells

被引：169

作者：

Lima, RT

Martins, SM

Guimaraes, JE

Sambade, C

Vasconcelos, MH

机构：

[1] Univ Porto, IPATIMUP, P-4200465 Oporto, Portugal

[2] Univ Porto, Fac Med, P-4100 Oporto, Portugal

[3] Canc Res UK, London, England

[4] Hosp Sao Joao, Oporto, Portugal

[5] Univ Porto, Fac Pharm, Dept Microbiol, P-4100 Oporto, Portugal

来源：

CANCER GENE THERAPY | 2004年 / 11卷 / 05期

关键词：

siRNAs; RNAi; bcl-2; xIAP;

D O I：

10.1038/sj.cgt.7700706

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Antiapoptotic genes such as bcl-2 or xlAP may be responsible for resistance to apoptosis induced by cytotoxic drugs. The aim of this study was to investigate if downregulation of bcl-2 or xlAP by RNA interference (RNAi) would sensitize MCF-7 cells to etoposide and doxorubicin. FlTC-siRNAs uptake was verified by fluorescence microscopy and downregulation of Bcl-2 or XlAP was confirmed by Western Blotting. Both siRNAs reduced the number of viable cells and increased cellular apoptosis. Treatment with siRNAs followed by treatment with etoposide or doxorubicin further reduced the number of viable cells, when compared to either of the treatments alone. Therefore, downregulation of bcl-2 or xlAP by RNAi enhances the effects of etoposide and doxorubicin.

引用

页码：309 / 316

页数：8

共 46 条

[1] Aryl hydrocarbon receptor gene silencing with small inhibitory RNA differentially modulates Ah-responsiveness in MCF-7 and HepG2 cancer cells [J].