1,25-dihydroxycholecalciferol regulates rat intestinal calbindin D-9k posttranscriptionally

To determine whether calbindin D-9k (CaBP) is subject to posttranscriptional control, 6-wk-old Sprague Dawley-derived rats were fed one of three purified diets, 1.5% Ca and 3.0% Ca, mostly as carbonate, and 2.9% Ca, mostly as gluconate. Two weeks later, 5-cm segments of duodenum, jejunum, ileum, cecum and colon were obtained and analyzed for CaBP and CaBP-mRNA. Analysis of the steady-state distribution of CaBP-mRNA and of CaBP revealed a statistically significant (r = 0.95; P < 0.01) linear relationship between CaBP-mRNA and CaBP. When, however, animals that had been fed the 1.5% Ca diet received by intrajugular injection 1.2 nmol 1,25-dihydroxycholecalciferol [1,25-(OH)(2)-D-3] and their CaBP-mRNA and CaBP were analyzed as a function of time after 1,25-(OH)(2)-D-3 administration, the kinetic response of the two molecules differed. The CaBP-mRNA increased linearly by similar to 68% for 4 h after administration and then declined over the next 6 h to a concentration below the preinjection value. Thus, appearance and disappearance of CaBP-mRNA approximated 17%. h(-1). The CaBP, however, increased steeply to 80% above preinjection concentration until 2 h postinjection, i.e., at a rate of 40%. h(-1). Thereafter, CaBP decreased to 35% above the preinjection value between 5 and 10 h postinjection (2.5%. h(-1)). These findings are consistent with a 1,25-(OH)(2)-D-3-mediated posttranscriptional regulation of CaBP concentrations, because the 1,25-(OH)(2)-D-3-mediated increase in CaBP-mRNA is not reflected in an immediately changed CaBP level.