Quantitation of protein on gels and blots by infrared fluorescence of Coornassie blue and Fast Green

被引：89

作者：

Luo, S ^{[1
]}

Wehr, NB ^{[1
]}

Levine, RL ^{[1
]}

机构：

[1] NHLBI, Biochem Lab, Bethesda, MD 20892 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2006年 / 350卷 / 02期

关键词：

Coomassie blue; protein quantitation; infrared fluorescence; Fast Green;

D O I：

10.1016/j.ab.2005.10.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this property to develop a rapid and sensitive method for detection and quantitation of proteins in gels and oil blots. The fluorescence response is quantitative for protein content between 10 ng and 20 mu g per band or spot. Staining and destaining require only 30 min, and the method is compatible with subsequent immunodetection. Published by Elsevier Inc.

引用

页码：233 / 238

页数：6

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