Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar typhimurium with macrophages and complement

被引:138
作者
Murray, GL
Attridge, SR
Morona, R [1 ]
机构
[1] Univ Adelaide, Sch Mol & Biomed Sci, Australian Bacterial Pathogenesis Program, Discipline Microbiol & Immunol, Adelaide, SA 5005, Australia
[2] Monash Univ, Dept Microbiol, Australian Bacterial Pathogenesis Program, Clayton, Vic 3800, Australia
[3] Univ Gothenburg, Dept Med Microbiol & Immunol, S-40530 Gothenburg, Sweden
关键词
D O I
10.1128/JB.188.7.2735-2739.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A panel of isogenic Salmonella enterica serovar Typhimurium strains that vary only in the length of the O antigen was constructed through complementation of a wzz double mutant (displaying unregulated O-antigen length) with ;one of two homologous (wzz(ST) and wzz(fepE)) or three heterologous (wzz(O139) of Vibrio cholerae and wzz(SF), and wzz(pHS-2) of Shigella flexneri) wzz genes. Each gene was functional in the S. enterica serovar Typhimurium host and specified production of O-antigen polymers with lengths typical of those synthesized by the donor bacteria (ranging from 2 to > 100 O-antigen repeat units). By use of this panel of strains, it was found that O-antigen length influences invasion/uptake by macrophage cells; this is the first time this has been shown with Salmonella. O-antigen length was confirmed to be related to complement resistance, with a minimum protective length of > 4 and < 15 repeat units. O antigen of 16 to 35 repeat units was found to activate complement more efficiently than other lengths, but this was unrelated to complement resistance. No evidence was found to suggest that modifying the length of the O-antigen polymer affected expression of the O1, O4, or I5 antigenic factors.
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页码:2735 / 2739
页数:5
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