Development and Characterization of Lactoferrin Loaded Poly(ε-Caprolactone) Nanofibers

被引：11

作者：

James, Eric N. ^{[1
,2
]}

Nair, Lakshmi S. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Orthoped Surg, Farmington, CT 06030 USA

[2] Univ Connecticut, Ctr Hlth, Inst Regenerat Engn, Farmington, CT 06030 USA

[3] Univ Connecticut, Ctr Hlth, Raymond Beverly Sackler Ctr Biomed Biol Phys & En, Farmington, CT 06030 USA

[4] Univ Connecticut, Dept Biomed Engn, Dept Mat Sci & Engn, Storrs, CT 06268 USA

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2014年 / 10卷 / 03期

关键词：

Lactoferrin; Extracellular Matrix (ECM); Polycaprolactone (PCL); Nanofiber; Tissue Engineering; ELECTROSPUN; SCAFFOLDS; GROWTH; MILK; DIFFERENTIATION; FABRICATION; PROTEIN;

D O I：

10.1166/jbn.2014.1717

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Lactoferrin loaded poly (epsilon-caprolactone) nanofibers were fabricated using the process of electrospinning and the osteo-compatibility of the scaffolds were evaluated using MC3T3-E1 osteoblast-like cells. Morphology of rhLF/PCL scaffolds was determined by scanning electron microscopy. Fourier transform infrared spectroscopy and Energy-dispersive X-ray spectroscopy confirmed the incorporation of rhLF in the PCL nanofibers. The surface distribution of rhLF on the nanofibers was evaluated using fluorescently tagged (Far-red) rhLF. The presence of rhLF on rhLF/PCL nanofibers significantly increased the proliferation and viability of MC3T3-E1 cells compared to cells seeded on PCL nanofibers as evidenced from Ki67 immuno-staining and MTT assay. The study demonstrated the feasibility of incorporating different concentrations of rhLF in PCL nanofibers and the potential of the fiber matrix to support osteoblast cell functions.

引用

页码：500 / 507

页数：8

共 40 条

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