Targeted disruption of the mouse Asna1 gene results in embryonic lethality

被引：42

作者：

Mukhopadhyay, Rita

Ho, Ye-Shih

Swiatek, Pamela J.

Rosen, Barry P.

Bhattacharjee, Hiranmoy

机构：

[1] Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI 48201 USA

[2] Wayne State Univ, Inst Environm Hlth Sci, Detroit, MI 48201 USA

[3] Van Andel Res Inst, Lab Germline Modificat, Grand Rapids, MI 49503 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 16期

关键词：

arsenic; ArsA; Asna1; embryonic lethality; knockout mouse;

D O I：

10.1016/j.febslet.2006.06.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bacterial ArsA ATPase is the catalytic component of an oxyanion pump that is responsible for resistance to arsenicals and antimonials. Homologues of the bacterial ArsA ATPase are widespread in nature. We had earlier identified the mouse homologue (Asna1) that exhibits 27% identity to the bacterial ArsA ATPase. To identify the physiological role of the protein, heterozygous Asnal knockout mice (Asna1(+/-)) were generated by homologous recombination. The Asna1(+/-) mice displayed similar phenotype as the wild-type mice. However, early embryonic lethality was observed in homozygous Asnal knockout embryos, between E3.5 (E = embryonic day) and E8.5 stage. These findings indicate that Asnal plays a crucial role during early embryonic development. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3889 / 3894

页数：6

共 24 条

[1]

Bhattacharjee H, 1999, SYMP SOC GEN MICROBI, V58, P58

[2] Genomic organization and chromosomal localization of the Asna1 gene, a mouse homologue of a bacterial arsenic-translocating ATPase gene [J].

Bhattacharjee, H ;

Ho, YS ;

Rosen, BP .

GENE, 2001, 272 (1-2) :291-299

[3] Structure-function relationships in an anion-translocating ATPase [J].

Bhattacharjee, H ;

Zhou, T ;

Li, J ;

Gatti, DL ;

Walmsley, AR ;

Rosen, BP .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2000, 28 :520-526

[4]

BRADLEY A, 1987, TERATOCARCINOMAS EMB, P113

[5] Chloride is an allosteric effector of copper assembly for the yeast multicopper oxidase Fet3p: An unexpected role for intracellular chloride channels [J].

Davis-Kaplan, SR ;

Askwith, CC ;

Bengtzen, AC ;

Radisky, D ;

Kaplan, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13641-13645

[6] Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae [J].

Dimmer, KS ;

Fritz, S ;

Fuchs, F ;

Messerschmitt, M ;

Weinbach, N ;

Neupert, W ;

Westermann, B .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (03) :847-853

[7]

Enyenihi AH, 2003, GENETICS, V163, P47

[8] The yeast CLC chloride channel functions in cation homeostasis [J].

Gaxiola, RA ;

Yuan, DS ;

Klausner, RD ;

Fink, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :4046-4050

[9] Functional profiling of the Saccharomyces cerevisiae genome [J].

Giaever, G ;

Chu, AM ;

Ni, L ;

Connelly, C ;

Riles, L ;

Véronneau, S ;

Dow, S ;

Lucau-Danila, A ;

Anderson, K ;

André, B ;

Arkin, AP ;

Astromoff, A ;

El Bakkoury, M ;

Bangham, R ;

Benito, R ;

Brachat, S ;

Campanaro, S ;

Curtiss, M ;

Davis, K ;

Deutschbauer, A ;

Entian, KD ;

Flaherty, P ;

Foury, F ;

Garfinkel, DJ ;

Gerstein, M ;

Gotte, D ;

Güldener, U ;

Hegemann, JH ;

Hempel, S ;

Herman, Z ;

Jaramillo, DF ;

Kelly, DE ;

Kelly, SL ;

Kötter, P ;

LaBonte, D ;

Lamb, DC ;

Lan, N ;

Liang, H ;

Liao, H ;

Liu, L ;

Luo, CY ;

Lussier, M ;

Mao, R ;

Menard, P ;

Ooi, SL ;

Revuelta, JL ;

Roberts, CJ ;

Rose, M ;

Ross-Macdonald, P ;

Scherens, B .

NATURE, 2002, 418 (6896) :387-391

[10] Functional discovery via a compendium of expression profiles [J].

Hughes, TR ;

Marton, MJ ;

Jones, AR ;

Roberts, CJ ;

Stoughton, R ;

Armour, CD ;

Bennett, HA ;

Coffey, E ;

Dai, HY ;

He, YDD ;

Kidd, MJ ;

King, AM ;

Meyer, MR ;

Slade, D ;

Lum, PY ;

Stepaniants, SB ;

Shoemaker, DD ;

Gachotte, D ;

Chakraburtty, K ;

Simon, J ;

Bard, M ;

Friend, SH .

CELL, 2000, 102 (01) :109-126

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