Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli

被引：211

作者：

Hill, Joshua ^{[1
]}

Beriwal, Shilpa ^{[1
]}

Chandra, Ishwad ^{[1
]}

Paul, Vinod K. ^{[2
]}

Kapil, Aarti ^{[3
]}

Singh, Tripti ^{[1
,5
]}

Wadowsky, Robert M. ^{[4
]}

Singh, Vinita

Goyal, Ankur ^{[1
]}

Jahnukainen, Timo ^{[1
]}

Johnson, James R. ^{[6
]}

Tarr, Phillip I. ^{[7
,8
]}

Vats, Abhay ^{[1
]}

机构：

[1] Childrens Hosp Pittsburgh, Dept Pediat, Pittsburgh, PA 15213 USA

[2] All India Inst Med Sci, Dept Pediat, New Delhi 110029, India

[3] All India Inst Med Sci, Dept Microbiol, New Delhi 110029, India

[4] Childrens Hosp Pittsburgh, Dept Pathol, Pittsburgh, PA 15213 USA

[5] Atharva BioSci, Dept Mol Diagnost, New Delhi 110029, India

[6] Univ Minnesota, Dept Med, Vet Affairs Med Ctr, VA Med Ctr, Minneapolis, MN 55417 USA

[7] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[8] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 08期

关键词：

D O I：

10.1128/JCM.00152-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples ( out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

引用

页码：2800 / 2804

页数：5

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