Bee pollen is an abundant source of high-quality triterpene alcohols and has been used as a phytomedicine treating benign prostatic hyperplasia. Free and esterified triterpene alcohols were extracted by n-hexane and separated by column chromatography. Comprehensive analysis of triterpene alcohols in bee pollen from six botanical origins in China was carried out by gas chromatography-mass spectrometry/flame ionization detector (GC-MS/FID). Six 4-desmethylsterols, one 4-monomethylsterol, four 4, 4-dimethylsterols and another three triterpene alcohols were reported for the first time in the bee pollen. Significant difference (P<0.05) was found in the contents of free and esterified triterpene alcohols between bee-collected pollens from the selected plant origins. The total triterpene alcohol levels in bee pollen oil fractions have been found to vary between 2.6 g/100 g and 20.4 g/100 g oil. Triterpene alcohols in the bee pollen oils were dominated by steryl esters (>67%). Concurrently, esterified triterpene alcohols in lotus bee pollen oil fraction predominantly consisted of large amount of cycloartenol (11.2 g/100 g oil). (C) 2012 Elsevier Ltd. All rights reserved.