A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

被引：11

作者：

Kim, JH ^{[1
]}

Kim, BG ^{[1
]}

Nam, H ^{[1
]}

Park, DE ^{[1
]}

Yun, KS ^{[1
]}

Yoon, JB ^{[1
]}

You, JC ^{[1
]}

Yoon, E ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Elect Engn & Comp Sci, Yusong Gu, Taejon 305701, South Korea

来源：

FIFTEENTH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST | 2002年

关键词：

D O I：

10.1109/MEMSYS.2002.984222

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purifiction chip, We have fabricated a microsieve type filter with an array of 2.2mum diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer, The fabricated DNA purification chip has shown a binding capacity of 15ng/cm(2) and a minimum extractable input concentration of 100copies/200muL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

引用

页码：133 / 136

页数：4

共 10 条

[1]

[Anonymous], [No title captured]

[2]

BELGRADER P, 2000, P MUTAS2000 NETH, P525

[3] Fast mixing by lamination. [J].

Branebjerg, J ;

Gravesen, P ;

Krog, JP ;

Nielsen, CR .

NINTH ANNUAL INTERNATIONAL WORKSHOP ON MICRO ELECTRO MECHANICAL SYSTEMS, IEEE PROCEEDINGS: AN INVESTIGATION OF MICRO STRUCTURES, SENSORS, ACTUATORS, MACHINES AND SYSTEMS, 1996, :441-446

[4] Modern genome research and DNA diagnostics in domestic animals in the light of classical breeding techniques [J].

Buitkamp, J ;

Epplen, JT .

ELECTROPHORESIS, 1996, 17 (01) :1-11

[5] A new monolithic micro biosensor for blood analysis [J].

Kim, JH ;

Kim, BG ;

Yoon, E ;

Han, CH .

14TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST, 2001, :443-446

[6] Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix [J].

Lakshmi, R ;

Baskar, V ;

Ranga, U .

ANALYTICAL BIOCHEMISTRY, 1999, 272 (01) :109-112

[7] Passive mixing in a three-dimensional serpentine microchannel [J].

Liu, RH ;

Stremler, MA ;

Sharp, KV ;

Olsen, MG ;

Santiago, JG ;

Adrian, RJ ;

Aref, H ;

Beebe, DJ .

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2000, 9 (02) :190-197

[8]

Manz A, 1998, MICROSYSTEM TECHNOLO

[9] Microfabricated devices for genetic diagnostics [J].

Mastrangelo, CH ;

Burns, MA ;

Burke, DT .

PROCEEDINGS OF THE IEEE, 1998, 86 (08) :1769-1787

[10] Driving forces for DNA adsorption to silica in perchlorate solutions [J].

Melzak, KA ;

Sherwood, CS ;

Turner, RFB ;

Haynes, CA .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 181 (02) :635-644

← 1 →