Brain derived neurotrophic factor induces a rapid upregulation of synaptophysin and tau proteins via the neurotrophin receptor TrkB in rat cerebellar granule cells

被引:47
作者
Coffey, ET
Akerman, KEO
Courtney, MJ
机构
[1] Dept. of Biochemistry and Pharmacy, Abo Akademi Univ., Biocity, P.O. B., Turku
基金
英国惠康基金; 芬兰科学院;
关键词
neurotrophin; brain derived neurotrophic factor; TrkB; tau; synaptophysin; cerebellar granule cells;
D O I
10.1016/S0304-3940(97)00335-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have examined the effects of neurotrophins brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) on the expression of the maturation-specific proteins synaptophysin end tau, and the growth-associated protein (GAP)-43 in cerebellar granule cells. We find that BDNF but not NGF rapidly (within 2 h) upregulates levels of synaptophysin, tau and c-Fos correlating with expression of the neurotrophin receptor TrkB. The rapid increase in synaptophysin is not preceded by c-Fos elevation suggesting a post-transcriptional mechanism may be involved. In contrast, no upregulation of GAP-43 levels are seen within this time period. Phorbol ester mimics the effects of BDNF, indicating that protein kinase C (PKC) is either a component of, or feeds into the signalling mechanism. We conclude that BDNF, characterized to be survival promoting early in differentiation of cerebellar granule cells, enhances maturation at a later stage. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:177 / 180
页数:4
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