A genetically encoded photosensitizer

被引：460

作者：

Bulina, ME

Chudakov, DM

Britanova, OV

Yanushevich, YG

Staroverov, DB

Chepurnykh, TV

Merzlyak, EM

Shkrob, MA

Lukyanov, S

Lukyanov, KA

机构：

[1] RAS, Inst Bioorgan Chem, Moscow 117997, Russia

[2] Evrogen JSC, Moscow 117997, Russia

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 01期

关键词：

D O I：

10.1038/nbt1175

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation(1). They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to beta-galactosidase and phospholipase C delta 1 pleckstrin homology domain.

引用

页码：95 / 99

页数：5

共 38 条

[1] New biarsenical Ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications [J].