NMR study of a Lewisx pentasaccharide derivative:: solution structure and interaction with cations

被引：36

作者：

Henry, B

Desvaux, H

Pristchepa, M

Berthault, P ^{[1
]}

Zhang, YM

Mallet, JM

Esnault, J

Sinaÿ, P

机构：

[1] CEA Saclay, Lab Commun RMN, DSM, DRECAM,SCM, F-91191 Gif Sur Yvette, France

[2] Ecole Normale Super, Dept Chim, CNRS, F-75231 Paris, France

来源：

CARBOHYDRATE RESEARCH | 1999年 / 315卷 / 1-2期

关键词：

Lewis(x); oligosaccharide; paramagnetic ions; calcium; H-1; NMR; C-13;

D O I：

10.1016/S0008-6215(98)00301-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure and conformation of the synthetic pentasaccharide Gal(beta 1-4){Fuc(alpha 1-3)}GlcNAc(beta 1-3)Gal(beta 1-4)Glc-beta OMe of the Lewis(x) family has been determined by NMR spectroscopy in dimethyl sulfoxide and methanol. In these solvents, the binding constants with calcium have been evaluated as 9.5 and 29.6 M-1, respectively. Study of the interaction sites has been achieved through the use of paramagnetic divalent cations and distance triangulation methods. Two regions have been found, the first one in the vicinity of the fucose unit, the second one closer to the lactose part. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：48 / 62

页数：15

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