Evaluation of a vanA-specific PCR assay for detection of vancomycin-resistant Enterococcus faecium during a hospital outbreak

被引:22
作者
Roger, M
Faucher, MC
Forest, P
St-Antoine, P
Coutlée, F
机构
[1] CHU Montreal, Dept Microbiol Med & Infectiol, Hop Notre Dame, Lab Epidemiol Mol, Montreal, PQ H2L 4M1, Canada
[2] CHU Montreal, Ctr Rech, Montreal, PQ, Canada
关键词
D O I
10.1128/JCM.37.10.3348-3349.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We investigated the use of PCR as an alternative to culture of fecal samples for detection of vanA-containing Enterococcus faecium during a recent hospital outbreak. Rectal swabs collected consecutively from 223 patients were analyzed by culture with and without enrichment broth and by vanA-specific PCR of enrichment broth samples. Fifty-five specimens were positive for vanA-containing E. faecium by at least one method. The sensitivities of the vanA-specific PCR assay and agar culture with and without enrichment broth were 94.5, 98, and 89%, respectively. All three methods were 100% specific. Final results were obtained much more rapidly by PCR (within 24 to 30 h of specimen submission) than by the culture methods (4 to 5 days). Thus, PCR is an accurate and rapid alternative to culture for detection of vancomycin-resistant enterococci during hospital outbreaks.
引用
收藏
页码:3348 / 3349
页数:2
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