Expression and localization of estrogen receptor-β in murine and human bone

被引:119
作者
Vidal, O
Kindblom, LG
Ohlsson, C
机构
[1] Sahlgrens Univ Hosp, Res Ctr Endocrinol & Metab, Dept Internal Med, Endocrine Bone Unit, S-41326 Gothenburg, Sweden
[2] Sahlgrens Univ Hosp, Dept Pathol, S-41345 Gothenburg, Sweden
关键词
D O I
10.1359/jbmr.1999.14.6.923
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Estrogens have profound effects on bone metabolism. Cellular responses to estrogens are mediated by estrogen receptors (ERs) which belong to the nuclear receptor superfamily. Two estrogen receptors, ER alpha and ER beta, have been cloned. Previously expression of ER alpha has been shown in osteoblasts. Here we demonstrate that the transcript for ER beta can be detected in the human osteosarcoma cell lines (MG-63 and SaOS-2) and in cultured human osteoblast-like cells. We also show that ER beta protein is present in nuclear extracts from these cells. Furthermore, ER beta immunoreactivity is found in sections of murine and human bone. Murine and human osteoblast and osteocyte nuclei are immunoreactive for ER beta. Osteoclasts are also ER beta immunoreactive but the staining is mainly cytoplasmic. The present study demonstrates that ER beta is present in all the cellular compartments involved in bone formation and bone resorption, both in human and in murine bone tissue.
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页码:923 / 929
页数:7
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